Yoshioka Yusuke, Kosaka Nobuyoshi, Konishi Yuki, Ohta Hideki, Okamoto Hiroyuki, Sonoda Hikaru, Nonaka Ryoji, Yamamoto Hirofumi, Ishii Hideshi, Mori Masaki, Furuta Koh, Nakajima Takeshi, Hayashi Hiroshi, Sugisaki Hajime, Higashimoto Hiroko, Kato Takashi, Takeshita Fumitaka, Ochiya Takahiro
1] Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan [2] Integrative Bioscience and Biomedical Engineering, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku, Tokyo 162-8480, Japan [3] Research Fellow of the Japan Society for the Promotion of Science (JSPS), Chiyoda-Ku, Tokyo 102-0083, Japan.
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.
Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.
癌细胞会向其微环境和循环系统中分泌小的膜性细胞外囊泡（EVs）。尽管它们作为癌症生物标志物的潜力很有前景，但在临床样本中鉴定和定量EVs仍然具有挑战性。在此，我们描述了一种灵敏且快速的分析技术，可直接从结直肠癌患者的血液样本中对循环EVs进行分析。EVs通过两种抗体捕获，并用光敏剂珠子进行检测，这使我们能够在无需纯化步骤的情况下检测癌症来源的EVs。我们还表明，使用嵌入癌症相关EVs中的抗原CD147，循环EVs可用于结直肠癌的检测。这项工作描述了一种灵敏检测疾病特异性循环EVs的新型液体活检技术，并从诊断和治疗的角度为转化医学提供了前景。
