Claeys Hannes, Van Landeghem Sofie, Dubois Marieke, Maleux Katrien, Inzé Dirk
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; andDepartment of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium; andDepartment of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
Plant Physiol. 2014 Jun;165(2):519-527. doi: 10.1104/pp.113.234641. Epub 2014 Apr 7.
In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and such studies thus only provide incomplete information about stress sensitivity in Arabidopsis (Arabidopsis thaliana). Similarly, transcript analysis revealed that typical stress markers are only induced at high stress levels in young seedlings. Therefore, tools are needed to study the effects of mild stress. We found that the commonly used stress-inducing agents mannitol, sorbitol, NaCl, and hydrogen peroxide impact shoot growth in a highly specific and dose-dependent way. Therefore, shoot growth is a sensitive, relevant, and easily measured phenotype to assess stress tolerance over a wide range of stress levels. Finally, our data suggest that care should be taken when using mannitol as an osmoticum.
体外胁迫试验常用于研究植物对非生物胁迫的响应并评估胁迫耐受性。一项文献综述表明，大多数研究使用非常高的胁迫水平，并测量诸如发芽、植物存活或白化等视觉症状的发展等标准。然而，我们表明这些参数是非常严重胁迫的指标，因此此类研究仅提供了关于拟南芥（Arabidopsis thaliana）胁迫敏感性的不完整信息。同样，转录分析表明，典型的胁迫标记仅在幼苗高水平胁迫下被诱导。因此，需要工具来研究轻度胁迫的影响。我们发现常用的胁迫诱导剂甘露醇、山梨醇、氯化钠和过氧化氢以高度特异性和剂量依赖性方式影响地上部生长。因此，地上部生长是一种敏感、相关且易于测量的表型，可用于评估广泛胁迫水平下的胁迫耐受性。最后，我们的数据表明在使用甘露醇作为渗透剂时应谨慎。
