Ishida Kelly, Cipriano Talita Ferreira, Rocha Gustavo Miranda, Weissmüller Gilberto, Gomes Fabio, Miranda Kildare, Rozental Sonia
Laboratório de Quimioterapia Antifúngica, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.
Laboratório de Biologia Celular de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro.
Mem Inst Oswaldo Cruz. 2014 Apr;109(2):220-8. doi: 10.1590/0074-0276130269.
The microbial synthesis of nanoparticles is a green chemistry approach that combines nanotechnology and microbial biotechnology. The aim of this study was to obtain silver nanoparticles (SNPs) using aqueous extract from the filamentous fungus Fusarium oxysporum as an alternative to chemical procedures and to evaluate its antifungal activity. SNPs production increased in a concentration-dependent way up to 1 mM silver nitrate until 30 days of reaction. Monodispersed and spherical SNPs were predominantly produced. After 60 days, it was possible to observe degenerated SNPs with in additional needle morphology. The SNPs showed a high antifungal activity against Candida and Cryptococcus , with minimum inhibitory concentration values ≤ 1.68 µg/mL for both genera. Morphological alterations of Cryptococcus neoformans treated with SNPs were observed such as disruption of the cell wall and cytoplasmic membrane and lost of the cytoplasm content. This work revealed that SNPs can be easily produced by F. oxysporum aqueous extracts and may be a feasible, low-cost, environmentally friendly method for generating stable and uniformly sized SNPs. Finally, we have demonstrated that these SNPs are active against pathogenic fungi, such as Candida and Cryptococcus.
纳米颗粒的微生物合成是一种将纳米技术与微生物生物技术相结合的绿色化学方法。本研究的目的是使用尖孢镰刀菌的水提取物来制备银纳米颗粒（SNP），以替代化学方法，并评估其抗真菌活性。在反应30天之前，SNP的产量以浓度依赖的方式增加，直至硝酸银浓度达到1 mM。主要产生单分散的球形SNP。60天后，可以观察到具有额外针状形态的退化SNP。SNP对念珠菌和隐球菌显示出高抗真菌活性，两个属的最低抑菌浓度值均≤1.68 µg/mL。观察到用SNP处理的新型隐球菌的形态学改变，如细胞壁和细胞质膜的破坏以及细胞质内容物的丧失。这项工作表明，尖孢镰刀菌水提取物可以轻松制备SNP，并且可能是一种可行、低成本、环境友好的方法，用于生成稳定且尺寸均匀的SNP。最后，我们证明了这些SNP对念珠菌和隐球菌等致病真菌具有活性。
