King S J, Sutton J G
Central Research Establishment, Home Office Forensic Science Service, Reading, Berkshire, U.K.
Forensic Sci Int. 1989 Mar;40(3):217-20. doi: 10.1016/0379-0738(89)90179-5.
The levels of 19-hydroxy-prostaglandins F1 alpha/F2 alpha (PG F) in the semen of 19 vasectomized, 44 infertile and 8 fertile men were determined using a simple RIA technique. The mean concentrations observed in this survey were 45 micrograms/ml, 49.5 micrograms/ml and 59 micrograms/ml, respectively. No significant difference was recorded between the vasectomized and infertile groups; there were too few fertile samples available to undertake a meaningful statistical comparison. No reduction was observed in the levels of this PG in a liquid semen sample retained at room temperature over a 4 week period in the presence of a bacteriostat (sodium azide). However, a 30% reduction in the levels of 19-OH PG F occurred over the same time period when aliquots of the same semen sample were retained at either room temperature or at 4 degrees C without azide. Finally, no reduction was observed in the concentration of 19-OH PG F in a series of 10-microliters semen stains stored over a period of 6 weeks at room temperature.
采用一种简单的放射免疫分析技术，测定了19名输精管切除术后男性、44名不育男性和8名可育男性精液中19-羟基前列腺素F1α/F2α（PG F）的水平。本次调查中观察到的平均浓度分别为45微克/毫升、49.5微克/毫升和59微克/毫升。输精管切除术后组和不育组之间未记录到显著差异；由于可育样本太少，无法进行有意义的统计比较。在有抑菌剂（叠氮化钠）存在的情况下，室温下保存4周的精液液体样本中，该PG的水平未观察到降低。然而，当相同精液样本的等分试样在室温或4℃下无叠氮化钠保存时，19-OH PG F的水平在同一时间段内降低了30%。最后，在室温下保存6周的一系列10微升精液污渍中，19-OH PG F的浓度未观察到降低。
