Sutton J G, King S J, Taylor M
Forensic Sci Int. 1987 May-Jun;34(1-2):143-53. doi: 10.1016/0379-0738(87)90092-2.
The specificity of the 19-OH F1 alpha/F2 alpha prostaglandin antisera for the detection of semen in seminal/vaginal mixtures, has been evaluated. Using a parallel curve test we found that the antibody showed a high specificity for these seminal prostaglandins in seminal/vaginal mixtures at concentrations of between 2 pg and 40 pg/100 microliter. The precision of the assay has been improved by the use of a donkey-anti-rabbit ferritin-bound second antibody. The application of this detection system makes it possible to complete an assay within 4.5 h. A survey of 50 semen-free vaginal swabs obtained from 3 donors, taken throughout the menstrual cycle, showed no trace of these prostaglandins. They were also not detected in the vaginal secretions of two further donors who were undergoing medication. Using only 10-microliter aliquots of a seminal/vaginal swab extract, prepared in 500 microliter, it was possible to detect semen up to approx. 80 h after one sexual act.
已对19-羟基F1α/F2α前列腺素抗血清在检测精液/阴道混合物中精液的特异性进行了评估。通过平行曲线试验，我们发现该抗体在精液/阴道混合物中，对于浓度在2 pg至40 pg/100微升之间的这些精液前列腺素表现出高特异性。通过使用驴抗兔铁蛋白结合二抗提高了检测的精密度。该检测系统的应用使得能够在4.5小时内完成一项检测。对从3名供体在整个月经周期采集的50份无精液阴道拭子进行的调查显示，未发现这些前列腺素的痕迹。在另外两名正在接受药物治疗的供体的阴道分泌物中也未检测到它们。仅使用在500微升中制备的精液/阴道拭子提取物的10微升等分试样，就能够在一次性行为后约80小时内检测到精液。
