King S J, Sutton J G, Trewsdale L A
Central Research Establishment, Home Office Forensic Science Service, Reading, Berkshire, U.K.
Forensic Sci Int. 1989 Mar;40(3):221-9. doi: 10.1016/0379-0738(89)90180-1.
The sensitivity of a simple radioimmunoassay (RIA) for the detection of 19-OH prostaglandin F1 alpha/F2 alpha (PG F) has been evaluated on a number of semen samples from vasectomized, infertile and fertile donors. The specificity of the test has been examined by testing saliva, sweat and urine from a number of male and female donors. The assay technique could readily detect the PG in semen stains prepared from 0.2 microliter of semen from normal, infertile and vasectomized donors. The detection limit of the assay system, based on the observed displacement, was calculated to be approximately 0.05 microliter semen. The assay could be conducted over a pH range of 7.5-10.5 even after the PG has been heated to 100 degrees C. The 19-OH series of PG were absent from sweat, saliva and female urine using the normal assay protocol; volumes in excess of 100 microliters of some urines particularly from women in labour and those with acute urinary tract infection showed some displacement. Low levels of PG were detected in 50% of the male urine analysed. However, urine samples from men who had engaged in recent sexual activity contained relatively high concentrations of PG which could be readily detected in 10 microliters of urine. These results emphasise the potential of these compounds as specific and sensitive markers for the presence of human semen.
已对来自输精管结扎者、不育和可育供体的大量精液样本评估了用于检测19-羟基前列腺素F1α/F2α（PG F）的简单放射免疫分析（RIA）的灵敏度。通过检测来自多名男性和女性供体的唾液、汗液和尿液来检验该检测的特异性。该分析技术能够轻松检测出由正常、不育和输精管结扎供体的0.2微升精液制备的精液污渍中的PG。根据观察到的置换情况计算，该分析系统的检测限约为0.05微升精液。即使PG已加热至100℃，该分析也可在pH值7.5至10.5的范围内进行。按照正常分析方案，汗液、唾液和女性尿液中不存在19-羟基系列的PG；某些尿液，特别是来自分娩期妇女和患有急性尿路感染的妇女的超过100微升的尿液量显示出一定程度的置换。在分析的50%男性尿液中检测到低水平的PG。然而，近期有过性活动的男性的尿液样本中含有相对较高浓度的PG，在10微升尿液中即可轻松检测到。这些结果强调了这些化合物作为人类精液存在的特异性和敏感标志物的潜力。
