Kelley K A, Pitha P M
Virology. 1985 Dec;147(2):382-93. doi: 10.1016/0042-6822(85)90140-0.
The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at low levels in poly rI.rC induced cells. MuIFN-beta 1, however, is expressed equally well in cells treated with poly rI.rC or infected with NDV. As shown by the use of a probe specific for poly rI.rC, interferon induction correlates with the cellular uptake of poly rI.rC into the cells. The relative levels of alpha and beta 1 mRNAs in the cells reached a maximum at 10 hr after the induction which indicates coordinate expression of alpha and beta 1 interferon genes. The effect of viral infection on the expression of two murine genes coinduced with interferon (pMIF20/11 and pMIF3/10) and several cellular genes was also examined. While pMIF20/11 is an inducible gene, the pMIF3/10 gene is expressed constitutively in mouse L cells. Viral infection, but not poly rI.rC treatment, enhanced the expression of the pMIF3/10 gene, as well as two other cellular genes; H-2 and c-myc, however, the expression of beta-actin gene was unaltered. These data indicate that enhancement of gene expression in virus infected cells in not limited to the interferon system.
聚胞苷酸（poly rI.rC）诱导的以及新城疫病毒（NDV）感染的小鼠细胞中，检测了I型鼠干扰素（MuIFN）基因和其他几个细胞基因的表达。对诱导的L细胞的RNA进行Northern分析显示，MuIFN-αs在NDV感染的细胞中高效表达，但在poly rI.rC诱导的细胞中仅低水平表达。然而，MuIFN-β1在经poly rI.rC处理或感染NDV的细胞中表达同样良好。如使用针对poly rI.rC的特异性探针所示，干扰素诱导与poly rI.rC进入细胞的摄取相关。诱导后10小时，细胞中α和β1 mRNA的相对水平达到最大值，这表明α和β1干扰素基因的协同表达。还检测了病毒感染对与干扰素共诱导的两个鼠基因（pMIF20/11和pMIF3/10）以及几个细胞基因表达的影响。虽然pMIF20/11是一个可诱导基因，但pMIF3/10基因在小鼠L细胞中组成性表达。病毒感染而非poly rI.rC处理增强了pMIF3/10基因以及其他两个细胞基因的表达；然而，H-2和c-myc基因以及β-肌动蛋白基因的表达未改变。这些数据表明，病毒感染细胞中基因表达的增强并不局限于干扰素系统。
