Bon Pierre, Aknoun Sherazade, Monneret Serge, Wattellier Benoit
Opt Express. 2014 Apr 7;22(7):8654-71. doi: 10.1364/OE.22.008654.
We describe the use of spatially incoherent illumination to make quantitative phase imaging of a semi-transparent sample, even out of the paraxial approximation. The image volume electromagnetic field is collected by scanning the image planes with a quadriwave lateral shearing interferometer, while the sample is spatially incoherently illuminated. In comparison to coherent quantitative phase measurements, incoherent illumination enriches the 3D collected spatial frequencies leading to 3D resolution increase (up to a factor 2). The image contrast loss introduced by the incoherent illumination is simulated and used to compensate the measurements. This restores the quantitative value of phase and intensity. Experimental contrast loss compensation and 3D resolution increase is presented using polystyrene and TiO(2) micro-beads. Our approach will be useful to make diffraction tomography reconstruction with a simplified setup.
我们描述了使用空间非相干照明对半透明样品进行定量相成像的方法，甚至超出了傍轴近似范围。通过使用四波横向剪切干涉仪扫描图像平面来收集图像体积内的电磁场，同时对样品进行空间非相干照明。与相干定量相测量相比，非相干照明丰富了收集到的三维空间频率，从而使三维分辨率提高（高达两倍）。模拟了由非相干照明引入的图像对比度损失，并用于补偿测量结果。这恢复了相位和强度的定量值。使用聚苯乙烯和TiO(2)微珠展示了实验性的对比度损失补偿和三维分辨率提高。我们的方法将有助于通过简化设置进行衍射层析成像重建。
