Sandoval-Denis Marcelo, Sutton Deanna A, Cano-Lira José F, Gené Josepa, Fothergill Annette W, Wiederhold Nathan P, Guarro Josep
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA.
J Clin Microbiol. 2014 Jun;52(6):2112-25. doi: 10.1128/JCM.00429-14. Epub 2014 Apr 9.
A set of 73 isolates of the emerging fungus Trichoderma isolated from human and animal clinical specimens were characterized morphologically and molecularly using a multilocus sequence analysis that included the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA and fragments of the translation elongation factor 1 alpha (Tef1), endochitinase CHI18-5 (Chi18-5), and actin 1 (Act1) genes. The most frequent species was Trichoderma longibrachiatum (26%), followed by Trichoderma citrinoviride (18%), the Hypocrea lixii/Trichoderma harzianum species complex (15%), the newly described species Trichoderma bissettii (12%), and Trichoderma orientale (11%). The most common anatomical sites of isolation in human clinical specimens were the respiratory tract (40%), followed by deep tissue (30%) and superficial tissues (26%), while all the animal-associated isolates were obtained from superficial tissue samples. Susceptibilities of the isolates to eight antifungal drugs in vitro showed mostly high MICs, except for voriconazole and the echinocandins.
从人类和动物临床标本中分离出一组73株新出现的木霉属真菌，利用多位点序列分析对其进行形态学和分子特征分析，该分析包括核糖体DNA的内部转录间隔区（ITS）以及翻译延伸因子1α（Tef1）、内切几丁质酶CHI18 - 5（Chi18 - 5）和肌动蛋白1（Act1）基因的片段。最常见的物种是长枝木霉（26%），其次是黄绿木霉（18%）、绿木霉/哈茨木霉复合种（15%）、新描述的物种比氏木霉（12%）和东方木霉（11%）。人类临床标本中最常见的分离解剖部位是呼吸道（40%），其次是深部组织（30%）和浅表组织（26%），而所有与动物相关的分离株均来自浅表组织样本。除伏立康唑和棘白菌素外，这些分离株对八种抗真菌药物的体外敏感性大多显示出高最低抑菌浓度。
