Aceti A, Celestino D, Teggi A, Caferro M, Pennica A, Grilli A, Sebastiani A, De Rosa F
Institute of the Clinic of Tropical and Infectious Diseases, La Sapienza University, Rome.
Clin Exp Allergy. 1989 May;19(3):335-9. doi: 10.1111/j.1365-2222.1989.tb02392.x.
An evaluation of the use of the histamine release test (HRT) in the diagnosis of human hydatidosis is presented. This technique, which makes it possible to evaluate directly IgE dependent immediate hypersensitivity by measuring the histamine released from leucocytes (basophils) after antigenic challenge, was compared with the detection of serum specific IgE by the radio-allergosorbent test (RAST), and with the determination of serum specific IgE by the enzyme-linked immunosorbent assay (ELISA). Of the 54 patients with hydatidosis, all were positive according to HRT, 42 according to RAST and 47 according to ELISA. No false positive results were obtained by HRT in 43 patients with parasitosis other than hydatidosis, however, of these 43, 10 resulted in false positives according to RAST and five according to ELISA. It is concluded that HRT is more sensitive and more specific than RAST and ELISA.
本文介绍了组胺释放试验（HRT）在人类包虫病诊断中的应用评估。该技术通过测量抗原激发后白细胞（嗜碱性粒细胞）释放的组胺，能够直接评估IgE依赖性速发型超敏反应，并与放射变应原吸附试验（RAST）检测血清特异性IgE以及酶联免疫吸附测定（ELISA）测定血清特异性IgE进行了比较。在54例包虫病患者中，HRT检测全部呈阳性，RAST检测42例呈阳性，ELISA检测47例呈阳性。在43例非包虫病寄生虫病患者中，HRT未获得假阳性结果，然而，在这43例患者中，RAST检测有10例假阳性，ELISA检测有5例假阳性。结论是，HRT比RAST和ELISA更敏感、更具特异性。
