Ye Jian, Zhu Rui, He Xiaosheng, Feng Yingying, Yang Liangle, Zhu Xiaoyan, Deng Qifei, Wu Tangchun, Zhang Xiaomin
Department of Occupational and Environmental Health and Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
PLoS One. 2014 Apr 10;9(4):e92964. doi: 10.1371/journal.pone.0092964. eCollection 2014.
Exposure to polycyclic aromatic hydrocarbons (PAHs) is associated with reduced heart rate variability (HRV), a strong predictor of cardiovascular diseases, but the mechanism is not well understood.
We hypothesized that PAHs might induce systemic inflammation and stress response, contributing to altered cardiac autonomic function.
HRV indices were measured using a 3-channel digital Holter monitor in 800 coke oven workers. Plasma levels of interleukin-6 (IL-6) and heat shock protein 70 (Hsp70) were determined using ELISA. Twelve urinary PAHs metabolites (OH-PAHs) were measured by gas chromatography-mass spectrometry.
We found that significant dose-dependent relationships between four urinary OH-PAHs and IL-6 (all Ptrend<0.05); and an increase in quartiles of IL-6 was significantly associated with a decrease in total power (TP) and low frequency (LF) (Ptrend = 0.014 and 0.006, respectively). In particular, elevated IL-6 was associated in a dose-dependent manner with decreased TP and LF in the high-PAHs metabolites groups (all Ptrend<0.05), but not in the low-PAHs metabolites groups. No significant association between Hsp70 and HRV in total population was found after multivariate adjustment. However, increased Hsp70 was significantly associated with elevated standard deviation of NN intervals (SDNN), TP and LF in the low-PAHs metabolites groups (all Ptrend<0.05). We also observed that both IL-6 and Hsp70 significantly interacted with multiple PAHs metabolites in relation to HRV.
In coke oven workers, increased IL-6 was associated with a dose-response decreased HRV in the high-PAHs metabolites groups, whereas increase of Hsp70 can result in significant dose-related increase in HRV in the low-PAHs metabolites groups.
接触多环芳烃（PAHs）与心率变异性（HRV）降低有关，HRV是心血管疾病的一个强有力的预测指标，但其机制尚不清楚。
我们假设PAHs可能会诱发全身炎症和应激反应，从而导致心脏自主神经功能改变。
使用三通道数字动态心电图监测仪对800名焦炉工人的HRV指标进行测量。采用酶联免疫吸附测定法测定血浆白细胞介素-6（IL-6）和热休克蛋白70（Hsp70）水平。通过气相色谱-质谱法测定12种尿中PAHs代谢物（OH-PAHs）。
我们发现四种尿中OH-PAHs与IL-6之间存在显著的剂量依赖关系（所有Ptrend<0.05）；IL-6四分位数的增加与总功率（TP）和低频（LF）的降低显著相关（Ptrend分别为0.014和0.006）。特别是，在高PAHs代谢物组中，IL-6升高与TP和LF降低呈剂量依赖关系（所有Ptrend<0.05），而在低PAHs代谢物组中则无此关系。多因素调整后，在总体人群中未发现Hsp70与HRV之间存在显著关联。然而，在低PAHs代谢物组中，Hsp70升高与正常心动周期标准差（SDNN）、TP和LF升高显著相关（所有Ptrend<0.05）。我们还观察到，IL-6和Hsp70在与HRV相关的多个PAHs代谢物方面均存在显著相互作用。
在焦炉工人中，高PAHs代谢物组中IL-6升高与HRV呈剂量反应性降低相关，而低PAHs代谢物组中Hsp70升高可导致HRV显著的剂量相关增加。
