Sprawka Iwona, Goławska Sylwia, Parzych Tina, Goławski Artur, Czerniewicz Paweł, Sytykiewicz Hubert
Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, ul. Prusa 12, 08-110 Siedlce, Poland
Department of Biochemistry and Molecular Biology, Siedlce University of Natural Sciences and Humanities, ul. Prusa 12, 08-110 Siedlce, Poland.
J Insect Sci. 2014 Jan 1;14. doi: 10.1093/jisesa/ieu094. Print 2014.
The toxicity effect of Concanavalin A (Canavalia ensiformis lectin, ConA) to bird cherry-oat aphid, Rhopalosiphum padi L. (Hemiptera: Aphididae), was investigated in the laboratory by using artificial diets containing ConA concentrations. Bird cherry-oat aphid performance was affected by the presence of Con A in artificial diets. The lectin added into the liquid diet increased the prereproductive period, mortality, and the average time of generation development (T) and decreased fecundity and the intrinsic rate of natural increase (rm). In attempt to unravel the mode of action of ConA, the interaction of the lectin with insect gut and the effect of ConA on feeding behavior were investigated. Extract of gut of treated grain aphid demonstrated DNA fragmentation, and this was accompanied with an increase in caspase 3 activity. Moreover, addition of ConA to the sucrose-agarose gels reduced salivation and passive ingestion of fluids from the gel. The results indicate that the insecticidal activity of ConA on R. padi may involve effects on death of the gut epithelial cells and effects on feeding behavior. This can be employed to create plants that are resistant to aphids.
通过使用含有不同浓度伴刀豆球蛋白A（刀豆凝集素，ConA）的人工饲料，在实验室中研究了伴刀豆球蛋白A对鸟害燕麦蚜（Rhopalosiphum padi L.，半翅目：蚜科）的毒性作用。人工饲料中ConA的存在影响了鸟害燕麦蚜的生长表现。添加到液体饲料中的凝集素延长了繁殖前期、增加了死亡率以及平均世代发育时间（T），并降低了繁殖力和内禀增长率（rm）。为了阐明ConA的作用模式，研究了凝集素与昆虫肠道的相互作用以及ConA对取食行为的影响。经处理的谷蚜肠道提取物显示出DNA片段化，同时伴随着半胱天冬酶3活性的增加。此外，向蔗糖-琼脂糖凝胶中添加ConA减少了唾液分泌以及从凝胶中被动摄取液体。结果表明，ConA对鸟害燕麦蚜的杀虫活性可能涉及对肠道上皮细胞死亡的影响以及对取食行为的影响。这可用于培育抗蚜虫的植物。
