埃及社区获得性肺炎儿童中IL6 - 174 G/C基因多态性及其与血清IL6的关系。
IL6-174 G/C gene polymorphism and its relation to serum IL6 in Egyptian children with community-acquired pneumonia.
作者信息
Zidan Haidy E, Elbehedy Rabab M, Azab Seham F
机构信息
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Pediatrics Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
出版信息
Cytokine. 2014 Jun;67(2):60-4. doi: 10.1016/j.cyto.2014.02.013. Epub 2014 Mar 24.
Cytokines are involved in the pathogenesis of community acquired pneumonia (CAP). The aim of this study is to investigate the association of IL6-174 G/C gene polymorphism with CAP in Egyptian children, to assess its effect on CAP outcome and to determine its effect on the serum IL6 levels in these children. IL6-174 G/C gene polymorphism was genotyped in 210 Egyptian children (100 patients with CAP and 110 healthy controls) using PCR-RFLP, while the serum IL6 levels were measured by ELISA method. We found a significant association between the GG genotype, G allele of IL6-174 G/C SNP and susceptibility to CAP (P=0.02, 0.01 respectively). However, GG genotype and G allele were protective against severe sepsis (p=0.004), acute respiratory failure (p<0.001) and hospital mortality (p<0.001). Serum IL6 levels were significantly increased in these children while there was no relation between GG genotype and serum IL6. In conclusion, IL6-174 G/C gene polymorphism may contribute to susceptibility to CAP in Egyptian children.
细胞因子参与社区获得性肺炎（CAP）的发病机制。本研究旨在探讨埃及儿童中白细胞介素6（IL6）-174 G/C基因多态性与CAP的相关性，评估其对CAP预后的影响，并确定其对这些儿童血清IL6水平的影响。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对210名埃及儿童（100例CAP患者和110名健康对照）进行IL6-174 G/C基因多态性基因分型，同时采用酶联免疫吸附测定（ELISA）法检测血清IL6水平。我们发现IL6-174 G/C单核苷酸多态性（SNP）的GG基因型、G等位基因与CAP易感性之间存在显著相关性（分别为P=0.02、0.01）。然而，GG基因型和G等位基因对严重脓毒症（p=0.004）、急性呼吸衰竭（p<0.001）和医院死亡率（p<0.001）具有保护作用。这些儿童的血清IL6水平显著升高，而GG基因型与血清IL6之间无相关性。总之，IL6-174 G/C基因多态性可能与埃及儿童CAP易感性有关。
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