Sousa Clara, Silva Liliana, Grosso Filipa, Lopes João, Peixe Luísa
REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
J Photochem Photobiol B. 2014 Apr 5;133:108-14. doi: 10.1016/j.jphotobiol.2014.02.015. Epub 2014 Mar 24.
In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n=148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.
在本研究中，我们开发并验证了一种基于傅里叶变换红外光谱衰减全反射（FTIR-ATR）的模型，用于对鲍曼不动杆菌临床分离株进行分型，这些分离株属于全球广泛传播的克隆复合体（CC）92中的ST98、ST208和ST218以及ST103。采集了77株先前已鉴定的分离株（多位点序列分型-MLST、脉冲场凝胶电泳-PFGE和碳青霉烯水解D类β-内酰胺酶-CHDL）的FTIR-ATR光谱，并通过偏最小二乘判别分析（PLSDA）进行建模。该模型用从不同国家和不同时期收集的、属于已建模和未建模ST的不同分离株（n=148）进行了测试，并成功验证。
