Tang H Z, Lu X F, Wang K J, Ouyang S, Huang Y Q
Department of Neurology, First Affiliated Hospital, Hunan Medical University, People's Republic of China.
Mol Chem Neuropathol. 1989 Apr;10(2):77-85. doi: 10.1007/BF03159715.
In order to understand the role of monoamines in cerebral ischemia, 3-methoxy-4-hydroxyphenylglycol(MHPG), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic acid(HVA), the three major unconjugated monoamine metabolites in cerebrospinal fluid (CSF), of 33 patients and 18 controls were measured with high performance liquid chromatography. Results showed all three metabolites were raised in patients with severe ischemia, but only MHPG and 5-HIAA were elevated significantly, MHPG changes more quickly and regularly as a consequence of cerebral ischemia than the two others. A positive correlation between any pair of metabolites was found in controls and in patients in the first week after stroke, but not at the end of the second week. Computer assisted multivariate analysis indicated 5-HIAA and MHPG correlated more closely with the state of illness in the acute stage, whereas HVA correlated the least. Possible explanations for the changes of CSF levels of amine metabolites are discussed.
为了了解单胺在脑缺血中的作用，采用高效液相色谱法测定了33例患者和18例对照者脑脊液（CSF）中三种主要的未结合单胺代谢产物，即3-甲氧基-4-羟基苯乙二醇（MHPG）、5-羟基吲哚乙酸（5-HIAA）和高香草酸（HVA）。结果显示，所有三种代谢产物在严重缺血患者中均升高，但只有MHPG和5-HIAA显著升高，与其他两种代谢产物相比，MHPG因脑缺血而变化更快且更有规律。在对照组和中风后第一周的患者中，发现任意两种代谢产物之间存在正相关，但在第二周结束时不存在。计算机辅助多变量分析表明，5-HIAA和MHPG在急性期与病情状态的相关性更密切，而HVA的相关性最小。文中讨论了胺代谢产物脑脊液水平变化的可能原因。
