Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
Department of Pharmaceutics and Biopharmacy, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
Biomed Res Int. 2014;2014:310680. doi: 10.1155/2014/310680. Epub 2014 Mar 4.
Deoxynivalenol (DON), produced by the Fusarium genus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2 mg/kg feed. The fish (n=40) were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225 μg/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964 μg/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH (P<0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol (P<0.05), and ammonia (P<0.01) was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol.
脱氧雪腐镰刀菌烯醇（DON）由镰刀菌属产生，是用于生产鱼饲料的谷物的主要污染物。采用添加 2mg/kg 饲料 DON 的商业饲料，研究了真菌毒素脱氧雪腐镰刀菌烯醇对虹鳟（Oncorhynchus mykiss）的影响。将鱼（n=40）暴露于真菌毒素 23 天。将鳜鱼分为两组，对照组和实验组。对照组饲喂天然污染低浓度 DON（225μg/kg 饲料）的商业饲料；实验组饲喂添加 DON（1964μg/kg 饲料）的商业饲料。实验结束时评估了血浆生化和血液学指标、生物计量参数和组织病理学变化。实验组的 MCH 值明显较低（P<0.05）。在生化指标方面，与对照组相比，实验组在 23 天暴露后，葡萄糖、胆固醇（P<0.05）和氨（P<0.01）显著降低。我们的评估显示，生物计量参数没有显著变化。组织病理学检查显示暴露鱼的尾肾紊乱。获得的数据表明，虹鳟（O. mykiss）对脱氧雪腐镰刀菌烯醇敏感。
