Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València València, Spain.
Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València València, Spain.
Front Plant Sci. 2014 Mar 27;5:115. doi: 10.3389/fpls.2014.00115. eCollection 2014.
It has been previously described that elevation of endogenous spermine levels in Arabidopsis could be achieved by transgenic overexpression of S-Adenosylmethionine decarboxylase (SAMDC) or Spermine synthase (SPMS). In both cases, spermine accumulation had an impact on the plant transcriptome, with up-regulation of a set of genes enriched in functional categories involved in defense-related processes against both biotic and abiotic stresses. In this work, the response of SAMDC1-overexpressing plants against bacterial and oomycete pathogens has been tested. The expression of several pathogen defense-related genes was induced in these plants as well as in wild type plants exposed to an exogenous supply of spermine. SAMDC1-overexpressing plants showed an increased tolerance to infection by Pseudomonas syringae and by Hyaloperonospora arabidopsidis. Both results add more evidence to the hypothesis that spermine plays a key role in plant resistance to biotic stress.
先前有研究描述，通过过表达 S-腺苷甲硫氨酸脱羧酶（SAMDC）或多胺合酶（SPMS），可以在拟南芥中提高内源腐胺水平。在这两种情况下，腐胺的积累都对植物转录组产生了影响，上调了一组在功能类别中富集的基因，这些基因参与了对生物和非生物胁迫的防御相关过程。在这项工作中，测试了过表达 SAMDC1 的植物对细菌和卵菌病原体的反应。在这些植物以及暴露于外源腐胺的野生型植物中，一些与病原体防御相关的基因的表达被诱导。过表达 SAMDC1 的植物对丁香假单胞菌和拟南芥疫霉的感染表现出更高的耐受性。这两个结果都为腐胺在植物对生物胁迫的抗性中发挥关键作用的假说提供了更多证据。
