de la Torre Eulalia, Colello Rocío, Padola Nora Lía, Etcheverría Analía, Rodríguez Edgardo, Amanto Fabián, Tapia María Ofelia, Soraci Alejandro Luis
Área de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
Área de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
Int J Microbiol. 2014;2014:489569. doi: 10.1155/2014/489569. Epub 2014 Mar 10.
Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.
整合子是参与获得抗生素耐药性的遗传元件之一。本研究的目的是调查从阿根廷一个农场生产系统不同阶段的猪以及环境中分离出的共生大肠杆菌菌株中整合子的存在情况。通过直肠拭子对5头产后母猪和每窝随机选取的5头仔猪进行采样。在第21天和第70天再次进行采样。还采集了该农场的环境样本。通过聚合酶链反应在100%的母猪以及生产不同阶段的仔猪中检测到含有任何整合子类或两种整合子组合的大肠杆菌：分娩栏阶段为68.1%；断奶时为60%，生长/育肥阶段为85.8%，显示出随着生产系统进程而增加。从环境样本中检测到78.4%含有任何整合子类的大肠杆菌。我们得出结论，动物和农场环境可作为通过整合子等移动遗传元件潜在传播耐药细菌的储存库，这对食用动物的生产有重大影响，并且可通过食物链传播给人类，构成公共卫生问题。
