Mhadhebi Lamia, Mhadhebi Amel, Robert Jacques, Bouraoui Abderrahman
Unit of Research of Bioactive Marine Substances (URSAM), Laboratory of Pharmacology, Faculty of Pharmacy of Monastir, Street Avicenne, 5000 Monastir, Tunisia. ; Laboratory of Pharmacology , University Victor Segalen Bordeaux 2, Institut Bergonié, 229 Street of Argonne, 33076 Bordeaux Cedex, France.
Laboratory of Chemistry, Faculty of Sciences of Monastir, Street of Environement, 5000 Monastir, Tunisia.
Iran J Pharm Res. 2014 Winter;13(1):207-20.
Seaweeds have caused an emerging interest in the biomedical area, mainly due to their contents of bioactive substances which show great potential as anti-inflammatory, anti-microbial, anti-viral and anti-tumoral drugs. Despite the diversity in quality and quantity of the Mediterranean Tunisian coast flora, with its large contains of marine organisms and seaweeds, most of them have not yet been investigated for pharmacological and biological activities. Antioxidant, anti-inflammatory and antiproliferative effects of the aqueous extracts (AQ) of three brown seaweed respectively, Cystoseira crinita (AQ-C cri), Cystoseira sedoides (AQ-C sed) and Cystoseira compressa (AQ-C com) were investigated. Antioxidant activity was evaluated using the DPPH assay. Total phenolic contents were measured using Folin-Ciocalteu method. The anti-inflammatory activity of these extracts was determined in-vivo, using carrageenan induced rat paw oedema assay. The antiproliferative activity was studied on normal cells (MDCK and rat fibroblast) and cancer (A549, MCF7 and HCT15) cell lines by the ability of the cells to metabolically reduce MTT formazan dyes, in comparison to a reference drug the Cisplatin. Results demonstrated that AQ-C cri, AQ-C sed and AQ-C com extracts exhibited significant radical scavenging activity. AQ-C com extract had the highest total phenolic content. AQ-C cri, AQ-C sed and AQ-C com extracts exhibited significant anti-inflammatory activity in a dose dependent manner by comparison to reference drugs. Moreover, AQ-C cri, AQ-C sed and AQ-C com extracts showed an important antiproliferative activity against both Human tumor cell lines HCT15 and MCF7. These pharmacological efficacies of these AQ- extracts of Cystoseira were positively correlated with their total phenol content and their good antioxidant activity. The purification and the determination of chemical structures of compounds of these active aqueous extracts are under investigation. It could have a promising role in the future medicine and nutrition when used as drug or food additive.
海藻已引起生物医学领域的新关注，主要是因其所含生物活性物质具有作为抗炎、抗菌、抗病毒和抗肿瘤药物的巨大潜力。尽管突尼斯地中海沿岸植物群在质量和数量上具有多样性，拥有大量海洋生物和海藻，但其中大多数尚未针对其药理和生物活性进行研究。分别对三种褐藻，即细弱囊链藻（AQ-C cri）、塞多伊斯囊链藻（AQ-C sed）和扁囊链藻（AQ-C com）的水提取物（AQ）的抗氧化、抗炎和抗增殖作用进行了研究。使用DPPH法评估抗氧化活性。采用福林-酚法测定总酚含量。这些提取物的抗炎活性通过角叉菜胶诱导的大鼠足爪水肿试验在体内进行测定。通过细胞代谢还原MTT甲臜染料的能力，与参考药物顺铂相比，研究了对正常细胞（MDCK和大鼠成纤维细胞）和癌细胞（A549、MCF7和HCT15）系的抗增殖活性。结果表明，AQ-C cri、AQ-C sed和AQ-C com提取物表现出显著的自由基清除活性。AQ-C com提取物的总酚含量最高。与参考药物相比，AQ-C cri、AQ-C sed和AQ-C com提取物以剂量依赖方式表现出显著的抗炎活性。此外，AQ-C cri、AQ-C sed和AQ-C com提取物对人肿瘤细胞系HCT15和MCF7均显示出重要的抗增殖活性。这些囊链藻AQ提取物的这些药理功效与其总酚含量及其良好的抗氧化活性呈正相关。这些活性水提取物化合物的纯化和化学结构测定正在进行中。当用作药物或食品添加剂时，它在未来医学和营养方面可能会发挥有前景的作用。
