Fjordbakk C T, Johansen G M, Løvås A C, Oppegård K L, Storset A K
Department of Companion Animal Clinical Sciences, Norwegian School of Veterinary Science, Oslo, Norway.
Equine Vet J. 2015 Mar;47(2):212-7. doi: 10.1111/evj.12277. Epub 2014 May 26.
No recommendations have been made regarding the relative timing of blood collection for autologous conditioned serum (ACS) preparation and surgical procedures.
Experimental in vitro study.
Blood was drawn from 15 stallions admitted for elective castration preoperatively and 22-24 h post operatively and incubated in ACS containers and plastic vacutainer tubes containing Z Serum Clot Activator. Concentrations of interleukin (IL)-1 receptor agonist (IL-1Ra), IL-10, IL-1β, tumour necrosis factor (TNF)-α, insulin-like growth factor (IGF)-1 and transforming growth factor (TGF)-β were determined in all serum samples and compared between preparation methods and sampling time by ANOVA. Changes in cytokine levels induced by incubation, defined as delta cytokine, were calculated by subtracting the baseline levels from the levels in incubated samples. Based on post operative serum amyloid A (SAA), horses were grouped into 'mild', moderate' and 'marked' surgical stress; delta cytokine levels in post operative samples were compared between these groups by ANOVA.
Delta IGF-1 was significantly lower in post operative samples compared with preoperative. Horses in the 'marked' surgical stress group had significantly lower delta IL-1Ra and delta TGF-β than the 'moderate' group and significantly lower delta IGF-1 than the 'mild' group. No association between cytokine levels and haematology variables were identified. Cytokine levels were comparable between serum prepared in blood tubes and in specialised ACS containers.
Surgical stress influences the cytokine content in ACS. Useful predictors of cytokine production in ACS were not identified. Specialised ACS containers may not be necessary for preparation of a cytokine-rich serum.
目前尚未就自体条件血清（ACS）制备过程中采血的相对时间与外科手术的关系给出相关建议。
1）确定手术应激对ACS中细胞因子水平的影响；2）确定用于预测ACS中细胞因子产生的血液学标志物；3）研究在制备富含细胞因子的血清时使用专用ACS容器的必要性。
体外实验研究。
采集15匹因择期去势而入院的种马术前及术后22 - 24小时的血液，分别在ACS容器以及含有Z血清凝块激活剂的塑料真空采血管中孵育。测定所有血清样本中白细胞介素（IL）-1受体拮抗剂（IL-1Ra）、IL-10、IL-1β、肿瘤坏死因子（TNF）-α、胰岛素样生长因子（IGF）-1和转化生长因子（TGF）-β的浓度，并通过方差分析比较制备方法和采样时间之间的差异。通过将孵育样本中的水平减去基线水平来计算孵育诱导的细胞因子水平变化，即细胞因子变化量（delta cytokine）。根据术后血清淀粉样蛋白A（SAA），将马匹分为“轻度”、“中度”和“重度”手术应激组；通过方差分析比较这些组术后样本中的细胞因子变化量水平。
与术前相比，术后样本中的IGF-1变化量显著降低。“重度”手术应激组的马匹IL-1Ra变化量和TGF-β变化量显著低于“中度”组，IGF-1变化量显著低于“轻度”组。未发现细胞因子水平与血液学变量之间存在关联。在血样管和专用ACS容器中制备的血清之间，细胞因子水平相当。
手术应激会影响ACS中的细胞因子含量。未发现ACS中细胞因子产生的有用预测指标。制备富含细胞因子的血清可能无需使用专用ACS容器。
