Ionita Christiane R, Troillet Antonia R, Vahlenkamp Thomas W, Winter Karsten, Brehm Walter, Ionita Jean-Claude
Am J Vet Res. 2016 Aug;77(8):898-905. doi: 10.2460/ajvr.77.8.898.
OBJECTIVE To compare humoral insulin-like growth factor (IGF)-1, platelet-derived growth factor (PDGF)-BB, transforming growth factor (TGF)-β1, and interleukin-1 receptor antagonist (IL-1Ra) concentrations in plasma and 3 types of equine autologous blood-derived preparations (ABPs). SAMPLE Blood and ABP samples from 12 horses. PROCEDURES Blood samples from each horse were processed by use of commercial systems to obtain plasma, platelet concentrate, conditioned serum, and aqueous platelet lysate. Half of the platelet concentrate samples were additionally treated with a detergent to release intracellular mediators. Humoral IGF-1, PDGF-BB, TGF-β1, and IL-1Ra concentrations were measured with ELISAs and compared statistically. RESULTS Median IGF-1 concentration was highest in conditioned serum and detergent-treated platelet concentrate, followed by platelet concentrate and plasma; IGF-1 was not detected in platelet lysate. Mean PDGF-BB concentration was highest in platelet lysate, followed by detergent-treated platelet concentrate and conditioned serum; PDGF-BB was not detected in plasma and platelet concentrate. Median TGF-β1 concentration was highest in detergent-treated platelet concentrate, followed by conditioned serum, platelet lysate, and platelet concentrate; TGF-β1 was not detected in most plasma samples. Median IL-1Ra concentration was highest in platelet lysate, followed by conditioned serum; IL-1Ra was not detected in almost all plasma, detergent-treated platelet concentrate, and platelet concentrate samples. CONCLUSIONS AND CLINICAL RELEVANCE Each ABP had its own cytokine profile, which was determined by the specific processing method. Coagulation and cellular lysis strongly increased humoral concentrations of cell-derived cytokines. No ABP had the highest concentrations for all cytokines. Further studies are needed to assess clinical relevance of these findings.
目的 比较血浆和3种马自体血源制剂（ABP）中体液胰岛素样生长因子（IGF）-1、血小板衍生生长因子（PDGF）-BB、转化生长因子（TGF）-β1和白细胞介素-1受体拮抗剂（IL-1Ra）的浓度。样本 来自12匹马的血液和ABP样本。方法 利用商业系统对每匹马的血液样本进行处理，以获得血浆、血小板浓缩物、条件血清和水性血小板裂解液。一半的血小板浓缩物样本另外用去污剂处理以释放细胞内介质。用酶联免疫吸附测定法（ELISA）测量体液中IGF-1、PDGF-BB、TGF-β1和IL-1Ra的浓度并进行统计学比较。结果 条件血清和经去污剂处理的血小板浓缩物中IGF-1浓度中位数最高，其次是血小板浓缩物和血浆；血小板裂解液中未检测到IGF-1。血小板裂解液中PDGF-BB平均浓度最高，其次是经去污剂处理的血小板浓缩物和条件血清；血浆和血小板浓缩物中未检测到PDGF-BB。经去污剂处理的血小板浓缩物中TGF-β1浓度中位数最高，其次是条件血清、血小板裂解液和血小板浓缩物；大多数血浆样本中未检测到TGF-β1。血小板裂解液中IL-1Ra浓度中位数最高，其次是条件血清；几乎所有血浆、经去污剂处理的血小板浓缩物和血小板浓缩物样本中均未检测到IL-1Ra。结论及临床意义 每种ABP都有其自身的细胞因子谱，这由特定的处理方法决定。凝血和细胞裂解强烈增加细胞衍生细胞因子的体液浓度。没有一种ABP对所有细胞因子的浓度都是最高的。需要进一步研究来评估这些发现的临床意义。
