登革2型（牙买加毒株1409）包膜蛋白中血清学定义的线性表位
Serologically defined linear epitopes in the envelope protein of dengue 2 (Jamaica strain 1409).
作者信息
Aaskov J G, Geysen H M, Mason T J
机构信息
Faculty of Health Science, Queensland University of Technology, Melbourne, Victoria, Australia.
出版信息
Arch Virol. 1989;105(3-4):209-21. doi: 10.1007/BF01311358.
Antisera from dengue patients and dengue virus infected rabbits recognized octapeptides corresponding to linear amino acid sequences in the envelope protein of dengue 2 (Jamaica 1409). Although no peptide was recognized by sera from all dengue infected hosts, two peptides (216LPLPWLPG223 and 448FSGVSWTM455) were recognized by sera from all dengue 2 infected rabbits. One of these 448FSGVSWTM455 was also recognized by sera from both the dengue 2 patients tested. No peptides were identified which reacted exclusively with all dengue 2 infected animals. Use of a mouse monoclonal antibody (1B7) enabled identification of two regions (50AKQPATLR57 and 127GKVVLPEN134) and possibly a third (349GRLITVNP356) in the envelope protein of dengue 2 likely to be involved in haemagglutination inhibition and virus neutralization in vitro.
登革热患者和登革热病毒感染兔子的抗血清识别出与登革热2型（牙买加1409）包膜蛋白线性氨基酸序列相对应的八肽。尽管并非所有登革热感染宿主的血清都能识别出肽段，但有两个肽段（216LPLPWLPG223和448FSGVSWTM455）能被所有登革热2型感染兔子的血清识别。其中448FSGVSWTM455肽段也能被所检测的两位登革热2型患者的血清识别。未鉴定出仅与所有登革热2型感染动物发生反应的肽段。使用小鼠单克隆抗体（1B7）能够鉴定出登革热2型包膜蛋白中可能参与体外血凝抑制和病毒中和的两个区域（50AKQPATLR57和127GKVVLPEN134）以及可能的第三个区域（349GRLITVNP356）。
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