Wang Jun, Ewing Andrew G
Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden.
Analyst. 2014 Jul 7;139(13):3290-5. doi: 10.1039/c4an00058g.
We report the application of individually addressable microelectrode arrays (MEAs) to study the heterogeneity of cell exocytosis at the subcellular level. Multiple subcellular-size electrodes are covered by a single PC12 cell for the investigation of subcellular exocytosis. PC12 cells have been seeded and cultured on top of three kinds of MEAs containing 16, 25, or 36 square microelectrodes (4 μm width in a 4 by 4 MEA, 3 μm width in a 5 by 5 MEA, 2 μm width in a 6 by 6 MEA). After collagen coating, single cells were found to cover several electrodes and these were selected for the study of subcellular exocytosis. Amperometric results show that single cell and subcellular heterogeneity in single cell exocytosis can be electrochemically detected with these MEAs. The results also show that these MEAs are suitable for detecting fast chemical events at single cells, as well as for developing multifunctional electrochemical sensors.
我们报告了可单独寻址的微电极阵列（MEA）在亚细胞水平研究细胞胞吐作用异质性方面的应用。单个PC12细胞覆盖多个亚细胞大小的电极，用于研究亚细胞胞吐作用。PC12细胞已接种并培养在三种含有16、25或36个方形微电极的MEA上（4×4 MEA中电极宽度为4μm，5×5 MEA中电极宽度为3μm，6×6 MEA中电极宽度为2μm）。胶原包被后，发现单个细胞覆盖了几个电极，并选择这些电极用于亚细胞胞吐作用的研究。安培测量结果表明，使用这些MEA可以电化学检测单细胞胞吐作用中的单细胞和亚细胞异质性。结果还表明，这些MEA适用于检测单细胞的快速化学事件，以及开发多功能电化学传感器。
