Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, 30100 Campus de Espinardo, Murcia, Spain.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2346-2352. doi: 10.1099/ijs.0.055095-0. Epub 2014 Apr 17.
Urolithins are dibenzopyranone metabolites that exert anti-inflammatory activity in vivo and are produced by the gut microbiota from the dietary polyphenols ellagic acid (EA) and ellagitannins. However, the bacteria involved in this process remain unknown. We report here a novel bacterium, strain CEBAS 1/15P(T), capable of metabolizing EA to urolithins, that was isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods. The strain was related to Gordonibacter pamelaeae 7-10-1-b(T), the type and only reported strain of the only species of the genus Gordonibacter, with about 97% 16S rRNA gene sequence similarity; they were both obligately anaerobic, non-spore-forming, Gram-stain-positive, short-rods/coccobacilli and metabolized only small numbers of carbon sources. L-Fucose, D-fructose, turanose, D-galacturonic acid and α-ketobutyric acid were metabolized by strain CEBAS 1/15P(T), while G. pamelaeae was negative for metabolism of these compounds. The whole-cell fatty acids consisted predominantly of saturated fatty acids (70%); strain CEBAS 1/15P(T) differed significantly from G. pamelaeae in the major fatty acid, which was C18 : 1ω9c, while anteiso-C15 : 0 was the major component for G. pamelaeae. The presence of a number of different fatty acid peaks, especially C19 : 0 cyclo and C18 : 1ω6c, was also indicative of distinct species. Six glycolipids (GL1-6) were recognized, while, in G. pamelaeae, only four glycolipids were described. On the basis of these data, the novel species Gordonibacter urolithinfaciens sp. nov. is described, with strain CEBAS 1/15P(T) ( = DSM 27213(T) = CCUG 64261(T)) as the type strain.
尿石素是二苯并吡喃酮代谢物，在体内具有抗炎活性，是肠道微生物群从饮食多酚鞣花酸（EA）和鞣花单宁产生的。然而，参与这一过程的细菌仍然未知。我们在这里报告一种新型细菌，菌株 CEBAS 1/15P（T），能够将 EA 代谢为尿石素，它是从健康人的粪便中分离出来的，并通过确定表型、生化和分子方法进行了特征描述。该菌株与 Gordonibacter pamelaeae 7-10-1-b（T）有关，后者是该属唯一物种 Gordonibacter pamelaeae 的模式和唯一报道的菌株，16S rRNA 基因序列相似度约为 97%；它们都是专性厌氧菌，不形成孢子，革兰氏阳性，短杆菌/短杆菌，只代谢少量碳源。菌株 CEBAS 1/15P（T）代谢 L-岩藻糖、D-果糖、棉子糖、D-半乳糖醛酸和 α-酮丁酸，而 G. pamelaeae 则不能代谢这些化合物。全细胞脂肪酸主要由饱和脂肪酸组成（70%）；菌株 CEBAS 1/15P（T）与 G. pamelaeae 在主要脂肪酸上有显著差异，后者是 C18：1ω9c，而 anteiso-C15：0 是 G. pamelaeae 的主要成分。存在许多不同的脂肪酸峰，特别是 C19：0 环和 C18：1ω6c，也表明是不同的物种。鉴定出 6 种糖脂（GL1-6），而 G. pamelaeae 仅描述了 4 种糖脂。基于这些数据，描述了一种新的物种 Gordonibacter urolithinfaciens sp. nov.，以菌株 CEBAS 1/15P（T）（ = DSM 27213（T） = CCUG 64261（T））作为模式菌株。
