Palatability pseudanthium 鲜叶提取物对 RAW264.7 巨噬细胞内 ROS 的清除活性和炎症介质的下调作用。
Intracellular ROS Scavenging Activity and Downregulation of Inflammatory Mediators in RAW264.7 Macrophage by Fresh Leaf Extracts of Pseuderanthemum palatiferum.
机构信息
School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
出版信息
Evid Based Complement Alternat Med. 2014;2014:309095. doi: 10.1155/2014/309095. Epub 2014 Mar 16.
Abstract
Beneficial antioxidant phytochemicals are found in many medicinal plants. Pseuderanthemum palatiferum (PP), a well-known Vietnamese traditional medicinal plant in Thailand, has long been used in folk medicine for curing inflammatory diseases, often with limited support of scientific research. Therefore, this study aimed to determine antioxidant and modulation of inflammatory mediators of ethanol and water extracts of PP (EEP and WEP, resp.). WEP had significantly higher phenolic and flavonoid levels and DPPH radical scavenging activity than EEP. However, EEP exhibited greater reducing power than WEP. A greater decrease of tert-butyl hydroperoxide-induced oxidative stress in RAW264.7 macrophage cells was also observed with EEP. Modulation of inflammatory mediators of EEP and WEP was evaluated on LPS plus IFN- γ -stimulated RAW264.7 cells. EEP more potently suppressed LPS plus IFN- γ -induced nitric oxide (NO) production than WEP. Both EEP and WEP also suppressed the expression of iNOS and COX-2 protein levels. Collectively, these results suggest that PP possesses strong antioxidant and anti-inflammatory properties.
摘要
许多药用植物中都含有有益的抗氧化植物化学物质。越南传统药用植物 Pseuderanthemum palatiferum（PP）在泰国早已被民间用于治疗炎症性疾病，但往往缺乏科学研究的支持。因此，本研究旨在确定 PP 的乙醇和水提取物（EEP 和 WEP）的抗氧化和调节炎症介质的特性。WEP 中的酚类和类黄酮含量以及 DPPH 自由基清除活性均显著高于 EEP。然而，EEP 的还原能力却大于 WEP。EEP 还能更大程度地降低 RAW264.7 巨噬细胞中叔丁基过氧化物诱导的氧化应激。我们还评估了 EEP 和 WEP 对 LPS 加 IFN-γ刺激的 RAW264.7 细胞中炎症介质的调节作用。与 WEP 相比，EEP 更能抑制 LPS 加 IFN-γ诱导的一氧化氮（NO）生成。EEP 和 WEP 还能抑制 iNOS 和 COX-2 蛋白水平的表达。综上所述，这些结果表明 PP 具有很强的抗氧化和抗炎特性。
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