Department of Food and Nutrition, Hannam University, Yuseonggu, Daejeon, Republic of Korea.
Toxicol In Vitro. 2011 Feb;25(1):138-44. doi: 10.1016/j.tiv.2010.10.005. Epub 2010 Oct 13.
Protective effects of quercetin and rutin against oxidative stress were evaluated using in vitro and intracellular antioxidant assay. Quercetin showed higher peroxyl and hydroxyl radical-scavenging activity in a dose-dependent manner than did rutin in oxygen-radical absorbance capacity (ORAC). At 10 and 100 μM, quercetin had higher metal-chelating activity than rutin carrying rutinose at position C-3 and was also more efficient than rutin in reducing intracellular oxidative stress caused by peroxyl radicals and Cu(2+). The protective activities of 10 and 100 μM quercetin against Cu(2+)-induced intracellular oxidation were 13.8% and 44.8%, respectively. Rutin showed no protective activity against Cu(2+)-induced oxidative stress. Quercetin showed significantly lower intracellular Cu(2+)-chelating activity than did 1,10-phenanthroline but offered greater protection from Cu(2+)-induced oxidative stress. Thus, quercetin may diffuse through the cell membrane more efficiently than rutin because quercetin does not carry rutinose, is hydrophilic, and reduces Cu(2+)-induced oxidative stress by scavenging radicals instead of chelating with metal ions.
采用体外和细胞内抗氧化测定法评价槲皮素和芦丁对氧化应激的保护作用。槲皮素在氧自由基吸收能力（ORAC）中比芦丁具有更高的过氧自由基和羟自由基清除活性，呈剂量依赖性。在 10 和 100 μM 时，槲皮素的金属螯合活性高于芦丁，因为芦丁在 C-3 位带有芦丁糖，并且比芦丁更有效地减少过氧自由基和 Cu(2+)引起的细胞内氧化应激。10 和 100 μM 槲皮素对 Cu(2+)-诱导的细胞内氧化的保护活性分别为 13.8%和 44.8%。芦丁对 Cu(2+)-诱导的氧化应激没有保护活性。与 1,10-菲咯啉相比，槲皮素的细胞内 Cu(2+)螯合活性显著降低，但提供了更大的保护，防止 Cu(2+)-诱导的氧化应激。因此，槲皮素可能比芦丁更有效地穿过细胞膜，因为槲皮素不携带芦丁糖，亲水性更强，并且通过清除自由基而不是与金属离子螯合来减少 Cu(2+)-诱导的氧化应激。
