Smirnov I V
Lab Delo. 1989(6):59-60.
The agglutination test in wells of polystyrene plates has been tried for the detection of virulent Yersinia. Glucose-peptone medium has been employed in the test; the results have been recorded in standard periods from the type of the precipitate. The method has been employed to detect the virulence plasmids in 138 strains of Y. enterocolitica, Y. frederiksenii, Y. kristensenii, Y. intermedia, Y. pseudotuberculosis, and to assess the heterogeneity of various populations of Yersinia in respect of this plasmid presence. Positive results of autoagglutination in the plates (with Y. pseudotuberculosis serovariants 03 and 09) as a rule correlated with Ca-dependence of the strains. The suggested method is not inferior to the tube method in its sensitivity and may be used in screening examinations.
已尝试采用聚苯乙烯板孔中的凝集试验来检测强毒力耶尔森菌。试验中使用了葡萄糖 - 蛋白胨培养基;根据沉淀类型在标准时间段记录结果。该方法已用于检测138株小肠结肠炎耶尔森菌、费氏耶尔森菌、克氏耶尔森菌、中间型耶尔森菌、假结核耶尔森菌中的毒力质粒,并评估不同耶尔森菌群体在该质粒存在方面的异质性。平板中自动凝集的阳性结果(假结核耶尔森菌血清变种03和09)通常与菌株的钙依赖性相关。所建议的方法在灵敏度上不低于试管法,可用于筛查检测。