Baishakhi Dey, Analava Mitra, School of Medical Science and Technology, IIT Kharaghpur 721302, India.
World J Diabetes. 2014 Apr 15;5(2):209-18. doi: 10.4239/wjd.v5.i2.209.
To investigate the presence and potency of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays.
The leaf extracts of the three different Eucalyptus species [E. globulus (EG), E. citriodora (EC), E. camaldulensis (ECA)] were subjected to in vitro assay procedures to explore the prevalence of natural enzyme inhibitors (NEIs) after preliminary qualitative and quantitative phytochemical evaluations, to study their inhibitory actions against the enzymes like α-amylase, α-glucosidase, aldose reductase, angiotensin converting enzyme and dipeptidyl peptidase 4 playing pathogenic roles in type 2 diabetes. The antioxidant potential and total antioxidant capacity of the species were also evaluated.
Major bioactive compounds like polyphenols (341.75 ± 3.63 to 496.85 ± 3.98) and flavonoids (4.89 ± 0.01 to 7.15 ± 0.02) were found in appreciable quantity in three species. Based on the IC50 values of the extracts under investigation, in all assays the effectivity was in the order of EG > ECA > EC. The results of the ferric reducing antioxidant power assay showed that the reducing ability of the species was also in the order of EG > ECA > EC. A strong correlation (R(2) = 0.81-0.99) was found between the phenolic contents and the inhibitory potentials of the extracts against the targeted enzymes.
These results show immense hypoglycemic potentiality of the Eucalyptus Spp. and a remarkable source of NEIs for a future phytotherapeutic approach in Type 2 diabetes.
通过体外试验研究不同桉树属植物（桉树属、桉树属、桉树属）叶提取物中具有降血糖潜力的天然酶抑制剂的存在和效力。
对三种不同桉树属植物（桉树属、桉树属、桉树属）的叶提取物进行体外试验程序，以探索天然酶抑制剂（NEIs）的存在，在初步定性和定量植物化学评价后，研究其对α-淀粉酶、α-葡萄糖苷酶、醛糖还原酶、血管紧张素转换酶和二肽基肽酶 4 的抑制作用，这些酶在 2 型糖尿病中起致病作用。还评估了物种的抗氧化潜力和总抗氧化能力。
在三种植物中发现了大量的生物活性化合物，如多酚（341.75±3.63 至 496.85±3.98）和类黄酮（4.89±0.01 至 7.15±0.02）。根据所研究提取物的 IC50 值，在所有试验中，桉树属的有效性均大于桉树属大于桉树属。铁还原抗氧化能力试验的结果表明，该物种的还原能力也桉树属大于桉树属大于桉树属。提取物对目标酶的抑制潜力与酚类含量之间存在很强的相关性（R2=0.81-0.99）。
这些结果表明桉树属具有巨大的降血糖潜力，是 2 型糖尿病未来植物治疗方法的天然酶抑制剂的重要来源。
