Mangeney M, Fischer A, Le Deist F, Latgé J P, Durandy A
U 132 INSERM, Hôpital des Enfants-Malades, Paris, France.
Cell Immunol. 1989 Sep;122(2):329-37. doi: 10.1016/0008-8749(89)90081-6.
We have studied the activation of human resting B cells by a carbohydrate antigen, mannan, with a polymannose branched repetitive structure. Mannan has been extracted from the cell wall of the Candida albicans yeast. For this purpose, dense G0 B lymphocytes were purified from tonsils. Mannan antigen was shown to trigger B cell activation, since an increase of cell volume and RNA synthesis occurred. B cell proliferation was observed following addition of recombinant interleukin 2, but not following addition of recombinant interleukin 4 or low-molecular-weight BCGF. The B cell activation appears to be mannan-specific since B cells obtained from mannan-sensitized subjects but not from unsensitized subjects were responsive. The observation that mannan antigen can directly activate specific dense B lymphocytes can be related to the previous observation that the in vitro anti-mannan antibody production does not require a cognate T-B cell interaction.
我们研究了一种具有多聚甘露糖分支重复结构的碳水化合物抗原——甘露聚糖对人静止B细胞的激活作用。甘露聚糖是从白色念珠菌酵母的细胞壁中提取的。为此，从扁桃体中纯化出密集的G0 B淋巴细胞。甘露聚糖抗原被证明能触发B细胞激活，因为细胞体积和RNA合成增加。添加重组白细胞介素2后观察到B细胞增殖，但添加重组白细胞介素4或低分子量B细胞生长因子后未观察到。B细胞激活似乎具有甘露聚糖特异性，因为从对甘露聚糖致敏的受试者而非未致敏的受试者获得的B细胞有反应。甘露聚糖抗原可直接激活特定的密集B淋巴细胞这一观察结果，可能与之前体外抗甘露聚糖抗体产生不需要同源T-B细胞相互作用的观察结果有关。
