Durandy A, Fischer A, Le Deist F, Drouhet E, Griscelli C
INSERM U 132, Hôpital Necker-Enfants Malades, Paris, France.
J Clin Immunol. 1987 Sep;7(5):400-9. doi: 10.1007/BF00917018.
We have studied T- and B-cell responses to antigens of Candida albicans in 18 patients suffering from chronic mucocutaneous candidiasis. We have shown that in vitro production of antibody to one of these antigens, mannan, was absent during the active phase of the disease and that this absence was consequent to the activation of specific CD8(+) and CD8(-) suppressor T lymphocytes. Such activation was also observed when control T lymphocytes were incubated in the presence of monocytes and a high concentration of mannan. This suppressive effect was specific to antigens of Candida albicans, was radiosensitive, and was not consequent to the secretion of prostaglandin E2. It appeared as well that the induction of these suppressor T cells was HLA-DQ restricted. The suppressor T-cell activity induced by antigens of Candida albicans in vitro is thus comparable to the suppressor T-cell activity observed in vivo in patients affected with chronic mucocutaneous candidiasis. Defective handling of mannan by monocytes could result in the accumulation of mannan, resulting in the activation of specific T suppressor cells and in the consequent cellular immunodeficiency specific to Candida albicans. Successful treatment of the candidiasis resulted in complete correction of the immune abnormalities.
我们研究了18例慢性黏膜皮肤念珠菌病患者对白色念珠菌抗原的T细胞和B细胞反应。我们发现，在疾病的活动期，针对其中一种抗原（甘露聚糖）的抗体在体外无法产生，而这种缺失是由于特异性CD8(+)和CD8(-)抑制性T淋巴细胞的激活所致。当对照T淋巴细胞在单核细胞和高浓度甘露聚糖存在的情况下孵育时，也观察到了这种激活。这种抑制作用对白色念珠菌抗原有特异性，对辐射敏感，且不是由前列腺素E2的分泌所致。此外，这些抑制性T细胞的诱导似乎受HLA-DQ限制。因此，白色念珠菌抗原在体外诱导的抑制性T细胞活性与慢性黏膜皮肤念珠菌病患者体内观察到的抑制性T细胞活性相当。单核细胞对甘露聚糖处理存在缺陷可能导致甘露聚糖积累，从而激活特异性T抑制细胞，并导致白色念珠菌特有的细胞免疫缺陷。念珠菌病的成功治疗导致免疫异常完全纠正。
