Tollemar J, Ringdén O, Holmberg K
Department of Clinical Immunology, Huddinge Hospital, Sweden.
Scand J Immunol. 1989 Oct;30(4):473-80. doi: 10.1111/j.1365-3083.1989.tb02452.x.
Challenging human lymphocytes with soluble cytoplasmic Candida albicans protein antigen (CP) and cell-wall-derived C. albicans mannan (CM) induced DNA synthesis and antibody secretion measured as plaque-forming cells (PFC) in an indirect haemolytic assay. Enriched T and B lymphocytes responded poorly to CP and CM. However, when adherent cells were added, T and B cells responded well to CM, but not to CP. Peripheral blood lymphocytes responded more strongly to CP than to CM, with peak stimulation on day 7. Bone marrow cells displayed similar DNA synthetic responses as blood lymphocytes, but peak stimulation occurred on days 4 and 5 for CP and CM, respectively. Cells from the various parenchymal organs, including adenoid, tonsil, and spleen, showed peak DNA synthesis ranging from days 3 to 5 with a tendency for CM to produce an earlier response than CP. In contrast to that observed in blood and bone marrow cells, CM induced a higher DNA synthesis in cells from parenchymal organs than CP, which gave low stimulations. PFC, like DNA synthesis, in blood and bone marrow cells responded best to CP, whereas spleen cells showed the highest response to CM. In blood and bone marrow cells, only IgG PFC were induced, but in spleen cells IgA and IgM PFC were also stimulated by CM. Fetal liver cells responded poorly to these two Candida preparations. CM but not CP stimulated DNA synthesis in umbilical cord blood lymphocytes and IgG, IgA, and IgM in spleen cells, suggesting that CM is a polyclonal activator and that CP acts as an antigen.
用可溶性白色念珠菌胞质蛋白抗原（CP）和细胞壁来源的白色念珠菌甘露聚糖（CM）刺激人淋巴细胞，在间接溶血试验中可诱导DNA合成，并以噬斑形成细胞（PFC）来衡量抗体分泌。富集的T淋巴细胞和B淋巴细胞对CP和CM反应不佳。然而，当加入黏附细胞时，T淋巴细胞和B淋巴细胞对CM反应良好，但对CP无反应。外周血淋巴细胞对CP的反应比对CM更强烈，在第7天达到刺激峰值。骨髓细胞显示出与血液淋巴细胞相似的DNA合成反应，但CP和CM的刺激峰值分别出现在第4天和第5天。来自包括腺样体、扁桃体和脾脏在内的各种实质器官的细胞，其DNA合成峰值出现在第3至5天，CM比CP有产生更早反应的趋势。与在血液和骨髓细胞中观察到的情况相反，CM在实质器官细胞中诱导的DNA合成高于CP，CP产生的刺激较低。PFC与DNA合成一样，血液和骨髓细胞中对CP反应最佳，而脾细胞对CM反应最高。在血液和骨髓细胞中，仅诱导产生IgG PFC，但在脾细胞中，CM也刺激产生IgA和IgM PFC。胎儿肝细胞对这两种念珠菌制剂反应不佳。CM而非CP刺激脐带血淋巴细胞中的DNA合成以及脾细胞中的IgG、IgA和IgM，这表明CM是一种多克隆激活剂，而CP作为一种抗原起作用。
