Departments of Neuroscience and Graduate Program in Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455.
J Neurosci. 2014 Apr 23;34(17):5895-908. doi: 10.1523/JNEUROSCI.0672-14.2014.
During development extrinsic guidance cues modulate the peripheral actin network in growth cones to direct axons to their targets. We wanted to understand the role of the actin nucleator Arp2/3 in growth cone actin dynamics and guidance. Since growth cones migrate in association with diverse adhesive substrates during development, we probed the hypothesis that the functional significance of Arp2/3 is substrate dependent. We report that Arp2/3 inhibition led to a reduction in the number of filopodia and growth cone F-actin content on laminin and L1. However, we found substrate-dependent differences in growth cone motility, actin retrograde flow, and guidance after Arp2/3 inhibition, suggesting that its role, and perhaps that of other actin binding proteins, in growth cone motility is substrate dependent.
在发育过程中，外部引导线索调节生长锥中的周围肌动蛋白网络，以将轴突引导至其靶标。我们想了解肌动蛋白核因子 Arp2/3 在生长锥肌动蛋白动力学和导向中的作用。由于生长锥在发育过程中与各种粘附底物一起迁移，因此我们探测了 Arp2/3 的功能意义是否取决于底物的假说。我们报告说，Arp2/3 抑制导致在层粘连蛋白和 L1 上的丝状伪足数量和生长锥 F-肌动蛋白含量减少。然而，我们发现 Arp2/3 抑制后生长锥运动、肌动蛋白逆行流动和导向存在底物依赖性差异，这表明其作用，也许还有其他肌动蛋白结合蛋白在生长锥运动中的作用，取决于底物。
