Arp2/3 在生长锥 actin 动力学和导向中的作用依赖于底物。
The role of Arp2/3 in growth cone actin dynamics and guidance is substrate dependent.
机构信息
Departments of Neuroscience and Graduate Program in Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455.
出版信息
J Neurosci. 2014 Apr 23;34(17):5895-908. doi: 10.1523/JNEUROSCI.0672-14.2014.
Abstract
During development extrinsic guidance cues modulate the peripheral actin network in growth cones to direct axons to their targets. We wanted to understand the role of the actin nucleator Arp2/3 in growth cone actin dynamics and guidance. Since growth cones migrate in association with diverse adhesive substrates during development, we probed the hypothesis that the functional significance of Arp2/3 is substrate dependent. We report that Arp2/3 inhibition led to a reduction in the number of filopodia and growth cone F-actin content on laminin and L1. However, we found substrate-dependent differences in growth cone motility, actin retrograde flow, and guidance after Arp2/3 inhibition, suggesting that its role, and perhaps that of other actin binding proteins, in growth cone motility is substrate dependent.
摘要
在发育过程中，外部引导线索调节生长锥中的周围肌动蛋白网络，以将轴突引导至其靶标。我们想了解肌动蛋白核因子 Arp2/3 在生长锥肌动蛋白动力学和导向中的作用。由于生长锥在发育过程中与各种粘附底物一起迁移，因此我们探测了 Arp2/3 的功能意义是否取决于底物的假说。我们报告说，Arp2/3 抑制导致在层粘连蛋白和 L1 上的丝状伪足数量和生长锥 F-肌动蛋白含量减少。然而，我们发现 Arp2/3 抑制后生长锥运动、肌动蛋白逆行流动和导向存在底物依赖性差异，这表明其作用，也许还有其他肌动蛋白结合蛋白在生长锥运动中的作用，取决于底物。
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