Hongal Sudhir, Torwane Nilesh Arjun, Goel Pankaj, Chandrashekar Byarakele
Department of Public Health Dentistry, People's Dental Academy, People's University, Bhopal, Madhya Pradesh, India.
Pharmacognosy Res. 2014 Apr;6(2):113-9. doi: 10.4103/0974-8490.129026.
This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse.
The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis.
GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface.
According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.
本研究比较了印度蜂胶30%乙醇提取物与含酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）的脱敏剂GC护齿泡沫封闭牙本质小管的能力。
从30颗新鲜拔除的完好人类第三磨牙制备标本，室温下保存在10%福尔马林（pH 7.0）中。从每个标本中获取一个切片样本（长5 mm×宽5 mm×深3.5 mm），包括颈部区域。样本用1000目和1200目氧化铝砂纸及金刚石糊剂进行打磨和湿抛光，以模拟临床牙本质敏感颈部表面情况。根据牙本质表面处理，将所有标本随机分为三组（n = 10）。阴性对照组：未处理标本（n = 4）和用6%柠檬酸预处理的标本（n = 6）；试验组：印度蜂胶30%乙醇提取物（n = 10）；阳性组：GC护齿泡沫（n = 10）。所有标本均制备用于扫描电子显微镜（SEM）分析。
GC护齿泡沫通过小管腔内的晶体样沉积物促进小管封闭。而蜂胶在牙本质表面形成一层薄而光滑的层。
根据SEM分析，两种脱敏剂均能封闭牙本质小管。
