Chen Jian, Qi Hui, Li Jin-Biao, Yi Yan-Qun, Chen Dan, Hu Xiao-Hong, Wang Mei-Ling, Sun Xing-Li, Wei Xiao-Yong
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):291-5.
To observe the effect and mechanism of Dendrobium candidum polysaccharides (DCP) in promoting hair growth, in order to lay a foundation for the development and utilization of D. candidum.
The water-extraction and alcohol-precipitation method was adopted to extract DCP, and the phenol-sulphuric acid method was used to determine its content. Thirty C57BL6J mice were collected to establish the hair loss model with hair removal cream. They were randomly divided into the control group, the positive control group and the DCP group, and given 0.2 mL of ultra-pure water, minoxidil tincture and DCP (5.0 g x L(-1)) 21 days. The mice hair growth scoring standard was adopted to evaluate the hair growth of C57BL/6J mice at 7, 14 d. The hairs in unit hair-losing areas of treated C57BL/6J mice at 21 d were weighed to evaluate the effect of DCP on the promotion of hair growth. MTT assay and RT-PCR method were used to evaluate the effect of DCP on the proliferatin of HaCaT cells and the mRNA expression of VEGF in HaCaT cells.
The extraction percent of DCP was 29.87%, and its content was 79.65%. The average scores for the hair growth and weight of C57BL/6J mice of DCP group were much higher than the control group. The survival rate and mRNA expression of VEGF of HaCaT cells were much higher than the control group.
DCP has the effect in promoting hair growth. Its mechanism may be related to the up-regulation of the mRNA expression of VEGF.
观察铁皮石斛多糖（DCP）促进毛发生长的作用及机制，为铁皮石斛的开发利用奠定基础。
采用水提醇沉法提取DCP，用苯酚-硫酸法测定其含量。选取30只C57BL6J小鼠，用脱毛膏建立脱发模型。将其随机分为对照组、阳性对照组和DCP组，分别给予0.2 mL超纯水、米诺地尔酊和DCP（5.0 g·L⁻¹），连续给药21天。采用小鼠毛发生长评分标准，于第7、14天评估C57BL/6J小鼠的毛发生长情况。于第21天称取处理后C57BL/6J小鼠单位脱毛区毛发重量，评估DCP促进毛发生长的效果。采用MTT法和RT-PCR法评估DCP对HaCaT细胞增殖及HaCaT细胞中VEGF mRNA表达的影响。
DCP提取率为29.87%，含量为79.65%。DCP组C57BL/6J小鼠的毛发生长评分及毛发重量平均值均显著高于对照组。HaCaT细胞的存活率及VEGF mRNA表达均显著高于对照组。
DCP具有促进毛发生长的作用。其机制可能与上调VEGF mRNA表达有关。
