Identification of Hair Growth Promoting Components in the Kernels of Koehne and Their Mechanism of Action.

The application of the seed oil of Koehne (Tibetan name ཁམབུ།), a plant belonging to the family, for the treatment of alopecia has been recorded in (ཤེལ་གོང་ཤེལ་ཕྲེང་།) (the classic of Tibetan medicine) and . This study aims to reveal the effective components and mechanism of hair growth promotion in the kernel of Koehne. Network pharmacology was used to predict the mechanism of action and effective components in the treatment of the kernel of Koehne. The contents of amygdalin in 12 batches of the kernel of Koehne were determined by HPLC. An animal model of the depilation of KM mice induced by sodium sulfide was created, and five effective components that promoted hair growth were initially screened. In the study of the effectiveness and mechanism of action, KM and C57BL/6 mice are selected as experimental objects, three screening tests for active components of the kernel of are performed, and three effective components are screened out from the eight components. HE staining was used to detect the number of hair follicles and the thickness of the dermis. RT-PCR and immunohistochemistry were used to evaluate the influence of the expression of indicators in the Wnt/β-catenin signaling pathway in skin, including β-catenin, GSK-3β, and mRNA and protein expression levels of Cyclin D 1 and LEF 1. The network pharmacology study showed 12 signaling pathways involving 25 targets in the treatment of alopecia by the kernel of Koehne. vitamin E (3.125 mg/cm/d), β-sitosterol (0.061 mg/cm/d), and linoleic acid (0.156 mg/cm/d) in the kernel of Koehne can promote hair growth in mice, and the mechanism of action may be related to the Wnt/β-catenin pathway.