Wen Jing, Qiao Ying, Xiong Zhili, Li Famei
a School of Pharmacy, Shenyang Pharmaceutical University , 110016 Shenyang , China.
Nat Prod Res. 2014;28(13):1025-9. doi: 10.1080/14786419.2014.909417. Epub 2014 Apr 25.
A simple and accurate HPLC-UV method was developed for simultaneous determination of seven constituents in Si-Ni-San decoction. Separation was performed on a Hypersil C18 column and detection was set with gradient wavelength at 240 nm (0-26 min) and 210 nm (26-30 min). The mobile phase consisted of 0.03% phosphoric acid in water (v/v), and acetonitrile was used with a flow rate of 1.0 (- 1). This method provides good linearity (r>0.9992), precision (RSD < 1.9%), repeatability (RSD < 2.9%), stability (RSD < 2.9%) and recovery (97.6-102.0%), which has been successfully applied to quantitative determination of the seven constituents in Si-Ni-San decoction and different compatibility groups.
建立了一种简单准确的高效液相色谱-紫外检测法，用于同时测定四逆散汤剂中的七种成分。采用Hypersil C18柱进行分离，检测波长采用梯度设置，在240 nm（0 - 26分钟）和210 nm（26 - 30分钟）。流动相由0.03%磷酸水溶液（v/v）组成，乙腈与之混合，流速为1.0（- 1）。该方法具有良好的线性关系（r > 0.9992）、精密度（RSD < 1.9%）、重复性（RSD < 2.9%）、稳定性（RSD < 2.9%）和回收率（97.6 - 102.0%），已成功应用于四逆散汤剂及其不同配伍组中七种成分的定量测定。
