Scroggs M W, Roggli V L, Fraire A E, Sanfilippo F
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Hum Pathol. 1989 Sep;20(9):845-9. doi: 10.1016/0046-8177(89)90095-6.
Intracytoplasmic globules have been described in a variety of neoplastic and nonneoplastic conditions, but remain poorly defined. In a review of 100 consecutive cases of lung carcinomas, six cases of mucin-positive adenocarcinoma demonstrated eosinophilic intracytoplasmic globules that ranged in size from less than 1 to 20 mu in diameter. The globules were often located adjacent to areas of tumor necrosis, and occurred either singly or multiply within individual tumor cells. Globules were similar in morphologic appearance to Russell bodies in plasma cells or the eosinophilic globules in hepatocytes of patients with alpha-1-antitrypsin deficiency, but were morphologically distinct from intracytoplasmic mucin vacuoles. The globules were brightly positive with PAS stain with diastase, were brick red with Masson's trichrome stain, and showed variably positive staining with Mallory's phosphotungstic acid-hematoxylin and Ziehl-Nielson stains. Immunoperoxidase staining showed slight staining of some globules with albumin, IgG, IgA, and alpha-1-antitrypsin. Ultrastructurally the globules had a homogeneous density and were often associated with profiles of rough endoplasmic reticulum. We suggest that these globules represent secretory glycoprotein accumulated in the cytoplasm of tumor cells in areas of tumor cell injury.
胞浆内小球已在多种肿瘤性和非肿瘤性疾病中被描述，但仍定义不清。在对100例连续肺癌病例的回顾中，6例黏液阳性腺癌显示有嗜酸性胞浆内小球，直径大小从小于1至20微米不等。这些小球常位于肿瘤坏死区域附近，在单个肿瘤细胞内可单个或多个出现。小球在形态学上类似于浆细胞中的拉塞尔小体或α1抗胰蛋白酶缺乏患者肝细胞中的嗜酸性小球，但在形态上与胞浆内黏液空泡不同。小球经淀粉酶消化后的PAS染色呈强阳性，经马松三色染色呈砖红色，经马洛里磷钨酸苏木精染色和齐尔-尼尔森染色呈不同程度的阳性。免疫过氧化物酶染色显示一些小球对白蛋白、IgG、IgA和α1抗胰蛋白酶有轻度染色。超微结构上，小球密度均匀一致，常与粗面内质网的形态相关。我们认为这些小球代表在肿瘤细胞损伤区域的肿瘤细胞质中积聚的分泌性糖蛋白。
