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Simultaneous application of in situ DNA hybridization and immunohistochemistry on one tissue section.

作者信息

Van der Loos C M, Volkers H H, Rook R, Van den Berg F M, Houthoff H J

机构信息

Department of Pathology, Academic Medical Centre, Amsterdam, The Netherlands.

出版信息

Histochem J. 1989 May;21(5):279-84. doi: 10.1007/BF01757180.

DOI:10.1007/BF01757180
PMID:2476418
Abstract

Combined application of a non-radioactive in situ DNA hybridization procedure and the immunoperoxidase technique on one tissue section is described. Of six potential protocols, only one proved to be successful. First, the immunohistochemical procedure including visualization of enzyme activity is performed; the in situ DNA hybridization protocol is then applied. Using this protocol, several antigens, detected with monoclonal antibodies, and target DNAs, detected by using biotinylated human cytomegalovirus or human papilloma virus type 16 DNA probes, could be distinguished by their peroxidase activity (brown precipitate) and alkaline phosphatase activity (purple-blue precipitate) respectively. The method allows immunophenotyping of virus-infected cells as well as simultaneous visualization of two viral parameters. This technique has important implications for research and diagnostic purposes.

摘要

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引用本文的文献

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Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.生物素标记多核苷酸的酶促合成：新型核酸亲和探针。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633-7. doi: 10.1073/pnas.78.11.6633.
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Simultaneous in situ detection of viral RNA and antigens.病毒RNA和抗原的同步原位检测。
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Paired indirect immunoenzyme staining with primary antibodies from the same species. Application of horseradish peroxidase and alkaline phosphatase as sequential labels.
Practical suggestions for successful immunoenzyme double-staining experiments.
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Interphase cytogenetics in paraffin sections of lung tumors by non-isotopic in situ hybridization. Mapping genotype/phenotype heterogeneity.通过非同位素原位杂交技术对肺肿瘤石蜡切片进行间期细胞遗传学分析。绘制基因型/表型异质性图谱。
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Detection of different developmental stages of malaria parasites by non-radioactive DNA in situ hybridization.通过非放射性DNA原位杂交检测疟原虫的不同发育阶段
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Simultaneous in situ genotyping and phenotyping of human papillomavirus cervical lesions: comparative sensitivity and specificity.人乳头瘤病毒宫颈病变的同步原位基因分型和表型分析：比较敏感性和特异性
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Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas.胰岛淀粉样多肽（IAPP）在胰岛中合成。通过原位杂交和免疫组化相结合的方法在大鼠胰腺中检测IAPP多肽和IAPP mRNA。
Histochemistry. 1992;97(1):33-7. doi: 10.1007/BF00271279.
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Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, non-fluorescent probes: technical considerations and biological implications.使用非同位素、非荧光探针在福尔马林固定的乳腺组织上进行染色体原位杂交：技术考量与生物学意义
Breast Cancer Res Treat. 1992;23(3):201-10. doi: 10.1007/BF01833516.
使用来自同一物种的一抗进行配对间接免疫酶染色。依次应用辣根过氧化物酶和碱性磷酸酶作为标记物。
Histochem J. 1984 May;16(5):477-87. doi: 10.1007/BF01041348.
4
Direct immunoenzyme double staining applicable for monoclonal antibodies.适用于单克隆抗体的直接免疫酶双重染色。
Histochemistry. 1984;80(2):103-6. doi: 10.1007/BF00679982.
5
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.用于可视化与固定在硝酸纤维素膜上的DNA或RNA杂交的生物素标记DNA探针的快速灵敏比色法：生物印迹法。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045-9. doi: 10.1073/pnas.80.13.4045.
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Comparison of paired immunofluorescence and paired immunoenzyme staining methods based on primary antisera from the same species.
J Histochem Cytochem. 1982 Jun;30(6):518-24. doi: 10.1177/30.6.6178779.
7
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段：一种新技术的超微结构细胞化学研究
J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
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Co-localization of corticotropin releasing factor and vasopressin mRNA in neurones after adrenalectomy.肾上腺切除术后促肾上腺皮质激素释放因子与血管加压素mRNA在神经元中的共定位
Nature. 1985;315(6014):59-61. doi: 10.1038/315059a0.
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Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.大鼠垂体同一组织切片中免疫细胞化学与原位杂交的联合应用。
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J Clin Pathol. 1987 Sep;40(9):1042-54. doi: 10.1136/jcp.40.9.1042.