Grupo de Física de Coloides y Polímeros, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Adv Healthc Mater. 2014 Aug;3(8):1309-25. doi: 10.1002/adhm.201400023. Epub 2014 Apr 25.
Here, the use of folic acid (FA)-functionalized, doxorubicin (DOXO)/superparamagnetic iron oxide nanoparticles (SPION)-loaded poly(lactic-co-glycolic acid) (PLGA)-Au porous shell nanoparticles (NPs) as potential nanoplatforms is reported for targeted multimodal chemo- and photothermal therapy combined with optical and magnetic resonance imaging in cancer. These polymeric-gold nanohybrids (PGNH) are produced by a seeded-growth method using chitosan as an electrostatic "glue" to attach Au seeds to DOXO/SPION-PLGA NPs. In order to determine their potential as theranostic nanoplatforms, their physicochemical properties, cellular uptake, and photothermal and chemotherapeutic efficiencies are tested in vitro using a human cervical cancer (HeLa) cell line. The present NPs show a near-infrared (NIR)-light-triggered release of cargo molecules under illumination and a great capacity to induce localized cell death in a well-focused region. The functionalization of the PGNH NPs with the targeting ligand FA improves their internalization efficiency and specificity. Furthermore, the possibility to guide the PGNH NPs to cancer cells by an external magnetic field is also proven in vitro, which additionally increases the cellular uptake and therapeutic efficiency.
本文报道了叶酸（FA）功能化、阿霉素（DOXO）/超顺磁性氧化铁纳米粒子（SPION）负载的聚乳酸-共-羟基乙酸（PLGA）-Au 多孔壳纳米粒子（NPs）作为潜在的纳米平台，用于癌症的靶向多模式化学和光热治疗，并结合光学和磁共振成像。这些聚合物-金纳米杂化物（PGNH）是通过种子生长法制备的，壳聚糖作为静电“胶”将 Au 种子附着到 DOXO/SPION-PLGA NPs 上。为了确定它们作为治疗性纳米平台的潜力，我们使用人宫颈癌（HeLa）细胞系在体外测试了它们的物理化学性质、细胞摄取以及光热和化学治疗效率。目前的 NPs 在光照下表现出近红外（NIR）光触发的货物分子释放，并且能够在聚焦区域内诱导局部细胞死亡。用靶向配体 FA 对 PGNH NPs 进行功能化，提高了它们的内化效率和特异性。此外，还在体外证明了通过外加磁场引导 PGNH NPs 到癌细胞的可能性，这进一步增加了细胞摄取和治疗效率。
