School of Forest and Resource Conservation, National Taiwan University , Taipei 10617, Taiwan.
J Agric Food Chem. 2014 May 7;62(18):4159-65. doi: 10.1021/jf500842v. Epub 2014 Apr 28.
The antioxidant activity and delayed aging effects of hot water extracts from leaves of Chamaecyparis obtusa var. formosana were investigated. Free radical, superoxide radical scavenging, and total phenolic content assays were employed to evaluate the in vitro activities of the extracts. In addition, in vivo assays using the nematode Caenorhabditis elegans were also performed in this study. The results showed that among all soluble fractions obtained from the extracts, the ethyl acetate-soluble fraction has the best in vitro and in vivo antioxidant activities. Moreover, it decreased significantly the deposition of lipofuscin (aging pigment) and extended the lifespan of C. elegans. Bioactivity-guided fractionation yielded six potent antioxidant constituents from the ethyl acetate-soluble fraction, namely, catechin, quercetin, quercetin-3-O-α-rhamnoyranoside, myricetin-3-O-α-rhamnoyranoside, vanillic acid, and 4-hydroxybenzoic acid. Quercetin-3-O-α-rhamnoyranoside pretreatment showed the highest survival of C. elegans upon juglone exposure. Taken together, the results revealed that hot water extracts from C. obtusa var. formosana leaves have the potential to be used as a source for antioxidant or delayed aging health food.
研究了台湾扁柏叶热水提取物的抗氧化活性和延缓衰老作用。采用自由基、超氧自由基清除和总酚含量测定法评价提取物的体外活性。此外，本研究还使用秀丽隐杆线虫进行了体内试验。结果表明，在所得到的所有可溶部分中，乙酸乙酯可溶部分具有最好的体外和体内抗氧化活性。此外，它显著降低脂褐素（衰老色素）的沉积并延长秀丽隐杆线虫的寿命。生物活性导向的分段得到了来自乙酸乙酯可溶部分的六种有效的抗氧化成分，分别是儿茶素、槲皮素、槲皮素-3-O-α-鼠李糖苷、杨梅素-3-O-α-鼠李糖苷、香草酸和 4-羟基苯甲酸。槲皮素-3-O-α-鼠李糖苷预处理在七叶树酮暴露时显示出秀丽隐杆线虫最高的存活率。总之，结果表明，台湾扁柏叶热水提取物具有作为抗氧化或延缓衰老保健食品的潜在用途。
