Delgado-Ruiz Rafael Arcesio, Calvo-Guirado José Luis, Romanos Georgios E
Department of Prosthodontics and Digital Technology, Stony Brook University, Stony Brook, NY, USA.
Faculty of Dentistry, Murcia University, Murcia, Spain.
Clin Oral Implants Res. 2015 Aug;26(8):915-930. doi: 10.1111/clr.12406. Epub 2014 Apr 26.
To perform a systematic review of studies that report the healing of critical size defects (CSDs) in rabbit calvaria and to determine the quality of the studies according to ARRIVE guidelines.
An Internet search was made in duplicate between December 2011 and August 2013 using MEDLINE, PubMed and Google Scholar (without restrictions on date of publication) for rabbit studies reporting the healing of CSD in the calvaria. Animal Research Reporting in Vivo Experiment (ARRIVE) guidelines (a list of 20 aspects to score and to ensure comparison between different experimental studies in animals) were used to evaluate the quality of the selected works.
Twenty-five manuscripts were evaluated. Case-control studies predominated (92.59%). Animal age was not stated in 70.37% of the studies; weight was not reported in 29.62%; most animals weighed 3.5 kg (26.31%). A CSD dimension of 15 mm was common (51.61%), generally located centrally (51.85%), followed by bilateral locations (48.14%). Circular (66.66%), rectangular (14.81%), square (14.81%) and ovoid (1.48%) geometries were used. Histomorphometric data showed incomplete healing in all CSDs and higher percentages of healing in smaller defects (<10 mm). The longer the healing time allowed, the more bone healing took place, for both smaller and larger defects (>15 mm). Minimum quality grades were assigned to ARRIVE items study design (6), experimental animals (8), housing and husbandry (9), sample size (10), allocation (11), statistics (13), results-baseline data (14), numbers analyzed (15), adverse events (17) and funding (20).
Data on CSDs in rabbit calvariae lack homogeneity. Smaller defects can be considered critical depending on the time of sacrifice. When new diagnostic technologies are used in addition to histomorphometry, these should be applied with caution to facilitate future comparison with other research. The ARRIVE guidelines should be followed in any animal research protocol to improve the homogeneity, comparison and reproducibility between studies.
对报告兔颅骨临界尺寸骨缺损（CSD）愈合情况的研究进行系统综述，并根据ARRIVE指南确定这些研究的质量。
于2011年12月至2013年8月期间，使用MEDLINE、PubMed和谷歌学术重复进行互联网搜索（不限制出版日期），查找报告兔颅骨CSD愈合情况的研究。使用动物体内实验研究报告（ARRIVE）指南（一份包含20个方面进行评分并确保不同动物实验研究之间具有可比性的清单）来评估所选研究的质量。
共评估了25篇手稿。病例对照研究占主导（92.59%）。70.37%的研究未说明动物年龄；29.62%未报告体重；大多数动物体重为3.5千克（26.31%）。15毫米的CSD尺寸较为常见（51.61%），通常位于中央（51.85%），其次是双侧位置（48.14%）。采用了圆形（66.66%）、矩形（14.81%）、方形（14.81%）和椭圆形（1.48%）的几何形状。组织形态计量学数据显示，所有CSD均愈合不完全，较小骨缺损（<10毫米）的愈合百分比更高。对于较小和较大骨缺损（>15毫米），允许的愈合时间越长，骨愈合发生得越多。对ARRIVE项目的研究设计（6分）、实验动物（8分）、饲养管理（9分）、样本量（10分）、分配（11分）、统计学（13分）、结果 - 基线数据（14分）、分析数量（15分）、不良事件（17分）和资金（20分）给予了最低质量等级。
兔颅骨CSD的数据缺乏同质性。根据处死时间的不同，较小的骨缺损也可被视为临界尺寸骨缺损。当除组织形态计量学外还使用新的诊断技术时，应谨慎应用，以便于未来与其他研究进行比较。在任何动物研究方案中都应遵循ARRIVE指南，以提高研究之间的同质性、可比性和可重复性。
