Geissler K, Tricot G, Grimm G, Siostrzonek P, Broxmeyer H
First Department of Medicine, University of Vienna, Austria.
Blut. 1989 Sep;59(3):226-30. doi: 10.1007/BF00320852.
Respiratory burst develops in myeloid blast cells if they differentiate functionally along the monocytic or granulocytic lineage. Using the nitroblue tetrazolium (NBT) assay we studied the effects of recombinant human granulocyte/macrophage colony stimulating factor (rhuGM-CSF), rhuG-CSF and rhuM-CSF on development of respiratory burst activity in primary blast cells from patients with myeloid leukemia. Assessing suspension cultures containing cells from patients with acute myeloid leukemia (AML, n = 13) or myeloid-blast crisis (myBC) of chronic myeloid leukemia (CML, n = 5) it was found that the percentage of NBT positive cells was increased by at least 20% as compared to control cultures by rhuGM-CSF in 6/17 cases, by rhuG-CSF in 7/17 cases and by rhuM-CSF in 0/16 cases, representing in 'responders' a mean increase of 267% and 270% in the absolute number of NBT positive cells by rhuGM-CSF and rhuG-CSF, respectively. Morphological examination of cultured cells from 'responders', as compared to controls, showed decreased blast cell content but generally no evidence of terminal differentiation. The demonstration of Auer rods in NBT positive cells indicates that respiratory burst developed in a leukemic clone. These findings may be of physiological, pathophysiological and clinical relevance.
如果髓系母细胞沿单核细胞或粒细胞谱系进行功能分化，就会发生呼吸爆发。我们使用硝基蓝四氮唑（NBT）试验，研究了重组人粒细胞/巨噬细胞集落刺激因子（rhuGM-CSF）、rhuG-CSF和rhuM-CSF对髓系白血病患者原代母细胞呼吸爆发活性发展的影响。评估含有急性髓系白血病（AML，n = 13）或慢性髓系白血病髓系母细胞危象（myBC，n = 5）患者细胞的悬浮培养物，发现与对照培养物相比，rhuGM-CSF使6/17例患者的NBT阳性细胞百分比增加至少20%，rhuG-CSF使7/17例患者的NBT阳性细胞百分比增加至少20%，而rhuM-CSF使0/16例患者的NBT阳性细胞百分比增加至少20%。在“反应者”中，rhuGM-CSF和rhuG-CSF使NBT阳性细胞绝对数量分别平均增加267%和270%。与对照相比，对“反应者”培养细胞进行形态学检查，发现母细胞含量减少，但一般没有终末分化的证据。在NBT阳性细胞中发现奥氏小体表明呼吸爆发发生在白血病克隆中。这些发现可能具有生理、病理生理和临床意义。
