Que Y, Xu L, Lin J, Chen R, Grisham M P
Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
United States Department of Agriculture-Agricultural Research Service, Sugarcane Research Unit, Houma, LA 70360.
Plant Dis. 2012 Oct;96(10):1519-1525. doi: 10.1094/PDIS-08-11-0663-RE.
Sugarcane smut caused by Sporisorium scitamineum occurs worldwide, causing serious losses in sugar yield and quality. To study the molecular variation of S. scitamineum, 23 S. scitamineum isolates collected from the six primary sugarcane production areas in mainland China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi provinces) were assessed by random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) markers. The results of RAPD, SRAP, and RAPD-SRAP combined analysis showed that, whereas the molecular variation of S. scitamineum was associated with geographic origin, there was no evidence of co-evolution between sugarcane and the pathogen. The results of RAPD, SRAP, or RAPD-SRAP combined analysis also did not provide any information about race differentiation of S. scitamineum. This suggests that the mixture of spores from sori collected from different areas should be used in artificial inoculations for resistance breeding and selection.
由甘蔗黑粉菌（Sporisorium scitamineum）引起的甘蔗黑穗病在全球范围内发生，导致甘蔗产量和品质严重受损。为研究甘蔗黑粉菌的分子变异情况，利用随机扩增多态性DNA（RAPD）和序列相关扩增多态性（SRAP）标记，对从中国大陆六个主要甘蔗产区（广西、云南、广东、海南、福建和江西省）收集的23株甘蔗黑粉菌分离株进行了评估。RAPD、SRAP及RAPD-SRAP联合分析结果表明，虽然甘蔗黑粉菌的分子变异与地理来源有关，但没有证据表明甘蔗与病原菌之间存在协同进化。RAPD、SRAP或RAPD-SRAP联合分析结果也未提供关于甘蔗黑粉菌生理小种分化的任何信息。这表明，在抗病育种和选择的人工接种中应使用从不同地区收集的孢子堆中的孢子混合物。
