Kouba Antonín, Velíšek Josef, Stará Alžběta, Masojídek Jiří, Kozák Pavel
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Department of Phototrophic Microorganisms, AlgaTech, Institute of Microbiology, Academy of Sciences, Opatovický mlýn, 379 81 Třeboň, Czech Republic.
Biomed Res Int. 2014;2014:408270. doi: 10.1155/2014/408270. Epub 2014 Feb 26.
Yearling common barbel (Barbus barbus L.) were fed four purified casein-based diets for 6 weeks in outdoor cages. Besides control diet, these were supplemented with 0.3 mg kg(-1) dw selenium (Se) from sodium selenite, or 0.3 and 1.0 mg kg(-1) from Se-enriched microalgae biomass (Chlorella), a previously untested Se source for fish. Fish mortality, growth, Se accumulation in muscle and liver, and activity of selected enzymes in blood plasma, muscle, liver, and intestine were evaluated. There was no mortality, and no differences in fish growth, among groups. Se concentrations in muscle and liver, activity of alanine aminotransferase and creatine kinase in blood plasma, glutathione reductase (GR) in muscle, and GR and catalase in muscle and liver suggested that selenium from Se-enriched Chlorella is more readily accumulated and biologically active while being less toxic than sodium selenite.
将一岁的普通鲃（Barbus barbus L.）在室外网箱中用四种以纯化酪蛋白为基础的饲料喂养6周。除了对照饲料外，其他饲料分别添加了0.3毫克/千克干重的亚硒酸钠硒（Se），或0.3和1.0毫克/千克的富含硒的微藻生物质（小球藻），这是一种此前未在鱼类中测试过的硒源。评估了鱼类死亡率、生长情况、肌肉和肝脏中的硒积累以及血浆、肌肉、肝脏和肠道中选定酶的活性。各组之间没有死亡率，鱼类生长也没有差异。肌肉和肝脏中的硒浓度、血浆中丙氨酸转氨酶和肌酸激酶的活性、肌肉中的谷胱甘肽还原酶（GR）以及肌肉和肝脏中的GR和过氧化氢酶表明，与亚硒酸钠相比，来自富含硒小球藻的硒更容易积累且具有生物活性，同时毒性更小。
