a Institute of Agricultural Sciences , University of the Punjab , Lahore , Pakistan.
Food Addit Contam Part B Surveill. 2014;7(1):57-62. doi: 10.1080/19393210.2013.843205. Epub 2013 Nov 5.
Aflatoxin and ochratoxin levels were determined in maize samples collected from store houses of 15 districts belonging to three agro-ecological zones of Punjab, Pakistan. Toxins were extracted by Aflaochra immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC). Mean moisture content of maize kernels was recorded above the safe storage level of 15%. Results indicated that aflatoxin B1 and B2 contamination was found in 97.3% and 78.9% of the collected samples, respectively. Aflatoxin G1, aflatoxin G2 and ochratoxin A were not detected in any sample. Among positive samples, 77.3% contained aflatoxin B1 and 28% aflatoxin B2, exceeding the legal limits as set by the European Union (EU) and the United States Food and Drug Administration (USFDA). It was concluded that a significant number of samples contained aflatoxin B1 and B2 above the legal limits.
从巴基斯坦旁遮普省三个农业生态区的 15 个地区的仓库中采集了玉米样本，检测了其中的黄曲霉毒素和赭曲霉毒素水平。毒素通过 Aflaochra 免疫亲和柱提取，并通过高效液相色谱（HPLC）进行分析。玉米籽粒的平均水分含量记录在 15%的安全储存水平以上。结果表明，所采集的样本中分别有 97.3%和 78.9%受到黄曲霉毒素 B1 和 B2 的污染。任何样本中均未检测到黄曲霉毒素 G1、黄曲霉毒素 G2 和赭曲霉毒素 A。在阳性样本中，77.3%含有黄曲霉毒素 B1，28%含有黄曲霉毒素 B2，超过了欧盟（EU）和美国食品和药物管理局（USFDA）设定的法定限量。结论是，相当数量的样本中黄曲霉毒素 B1 和 B2 的含量超过了法定限量。
