Department of Molecular and Cell Biology, University of Connecticut Storrs, CT, USA.
Department of Microbiology and Parasitology, University of Seville Seville, Spain.
Front Microbiol. 2014 Apr 9;5:143. doi: 10.3389/fmicb.2014.00143. eCollection 2014.
Halobacteria require high NaCl concentrations for growth and are the dominant inhabitants of hypersaline environments above 15% NaCl. They are well-documented to be highly recombinogenic, both in frequency and in the range of exchange partners. In this study, we examine the genetic and genomic variation of cultured, naturally co-occurring environmental populations of Halobacteria. Sequence data from multiple loci (~2500 bp) identified many closely and more distantly related strains belonging to the genera Halorubrum and Haloarcula. Genome fingerprinting using a random priming PCR amplification method to analyze these isolates revealed diverse banding patterns across each of the genera and surprisingly even for isolates that are identical at the nucleotide level for five protein coding sequenced loci. This variance in genome structure even between identical multilocus sequence analysis (MLSA) haplotypes indicates that accumulation of genomic variation is rapid: faster than the rate of third codon substitutions.
嗜盐菌需要高浓度的 NaCl 才能生长，是高于 15%NaCl 的高盐环境中的主要居民。它们具有高度的重组性，无论是在频率上还是在交换伙伴的范围上。在这项研究中，我们研究了培养的、自然共存的嗜盐菌环境种群的遗传和基因组变异。来自多个基因座（约 2500bp）的序列数据确定了许多属于 Halorubrum 和 Haloarcula 属的密切相关和更远缘的菌株。使用随机引物 PCR 扩增方法对这些分离物进行基因组指纹图谱分析，揭示了每个属之间以及甚至在五个蛋白质编码序列基因座的核苷酸水平上完全相同的分离物之间的多样化带型。即使在相同的多位点序列分析 (MLSA) 单倍型之间，基因组结构的这种差异也表明基因组变异的积累是迅速的：比第三密码子替换的速度还要快。
