Microbiology Graduate Group, University of California Davis, Davis, California, United States of America.
PLoS One. 2012;7(7):e41389. doi: 10.1371/journal.pone.0041389. Epub 2012 Jul 24.
We report the sequencing of seven genomes from two haloarchaeal genera, Haloferax and Haloarcula. Ease of cultivation and the existence of well-developed genetic and biochemical tools for several diverse haloarchaeal species make haloarchaea a model group for the study of archaeal biology. The unique physiological properties of these organisms also make them good candidates for novel enzyme discovery for biotechnological applications. Seven genomes were sequenced to ∼20×coverage and assembled to an average of 50 contigs (range 5 scaffolds-168 contigs). Comparisons of protein-coding gene compliments revealed large-scale differences in COG functional group enrichment between these genera. Analysis of genes encoding machinery for DNA metabolism reveals genera-specific expansions of the general transcription factor TATA binding protein as well as a history of extensive duplication and horizontal transfer of the proliferating cell nuclear antigen. Insights gained from this study emphasize the importance of haloarchaea for investigation of archaeal biology.
我们报告了来自两个盐杆菌属（Haloferax 和 Haloarcula）的 7 个基因组的测序。由于这些嗜盐古菌易于培养，并且存在针对多种不同嗜盐古菌物种的成熟遗传和生化工具，因此它们成为研究古菌生物学的模型群体。这些生物体独特的生理特性也使它们成为生物技术应用中新型酶发现的良好候选者。对七个基因组进行测序，覆盖率约为 20×，并组装成平均 50 个连续序列（范围为 5 个支架-168 个连续序列）。对编码蛋白质的基因进行比较，发现这两个属在 COG 功能组富集方面存在大规模差异。对 DNA 代谢相关基因的分析揭示了一般转录因子 TATA 结合蛋白的特定基因扩展，以及增殖细胞核抗原的广泛复制和水平转移的历史。从这项研究中获得的见解强调了嗜盐古菌在研究古菌生物学方面的重要性。
