Vineetha Ravindranath, Pai Keerthilatha-M, Vengal Manoj, Gopalakrishna Kodyalamoole, Narayanakurup Dinesh
MDS, Associate Professor. Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Manipal University. Karnataka State, India.
MDS, Professor and Head. Department of Oral Medicine and Radiology, Manipal College of Dental Sciences, Manipal University. Karnataka State, India.
J Clin Exp Dent. 2014 Apr 1;6(2):e132-7. doi: 10.4317/jced.51355. eCollection 2014 Apr.
Salivary biomarkers are suggested to provide a reliable, noninvasive and objective measurement of chronic psychosocial stress and helps in assessment of pivotal role of stress in causation or precipitation of multitude of health problems.
To evaluate the usefulness of salivary alpha amylase activity as an objective indicator of chronic stress and to find out any correlation between stress- related mucosal complaints and its levels.
Study was conducted among 50 subjects suffering from chronic stress related problems and 50 non-stressed individuals who were screened with a psychometric questionnaire. Brief case history and oral examination was carried out and about one ml of unstimulated saliva was collected. Salivary alpha amylase levels estimated were compared between study and control group and between subjects with and without oral mucosal changes using non parametric Mann Whitney U test.
There was statistically significant higher salivary alpha amylase levels in study group (p =.002) and salivary alpha amylase between the oral mucosal complaints group and without oral mucosal complaints group within the total study population were found to be statistically significant (p=0.045).
Salivary amylase activity increases in patients with chronic psychosocial stress and may be used as a biomarker of chronic stress, but it may not be an indicator to suggest the development of stress related oral mucosal changes. Key words:Salivary biomarker, salivary alpha amylase, psychosocial stress, sympathetic nervous system, oral mucosal changes.
唾液生物标志物被认为可以提供一种可靠、无创且客观的慢性心理社会应激测量方法，并有助于评估应激在多种健康问题的发生或诱发中所起的关键作用。
评估唾液α淀粉酶活性作为慢性应激客观指标的实用性，并找出应激相关口腔黏膜症状与其水平之间的相关性。
对50名患有慢性应激相关问题的受试者和50名通过心理测量问卷筛选出的无应激个体进行了研究。记录简要病史并进行口腔检查，收集约1毫升未刺激唾液。使用非参数曼-惠特尼U检验比较研究组和对照组之间以及有和无口腔黏膜变化的受试者之间的唾液α淀粉酶水平。
研究组的唾液α淀粉酶水平在统计学上显著更高（p = 0.002），并且在整个研究人群中，口腔黏膜症状组和无口腔黏膜症状组之间的唾液α淀粉酶水平在统计学上也显著不同（p = 0.045）。
慢性心理社会应激患者的唾液淀粉酶活性增加，可作为慢性应激的生物标志物，但它可能不是提示应激相关口腔黏膜变化发生的指标。关键词：唾液生物标志物、唾液α淀粉酶、心理社会应激、交感神经系统、口腔黏膜变化
