Gaurav Ashish, Sharma Gaganjot Kaur, Chhabra Ajay, Bamba Sakshi, Sahoo Sabashish, Chhabra Vandana
Department of Dentistry, AIIMS, Kalyani, West Bengal, India.
Department of Oral Pathology, Shaheed Kartar Singh Sarabha Dental College, Sarabha, Ludhiana, India.
J Oral Maxillofac Pathol. 2024 Apr-Jun;28(2):178-181. doi: 10.4103/jomfp.jomfp_462_23. Epub 2024 Jul 11.
Stress is faced by every individual in their day-to-day life activities. During pandemic, most people have experienced multiple episodes of depression, anxiety, and insomnia. Several medical and dental problems are reported with stress and the fastest and easiest way to study and investigate is through body fluids, mainly saliva. Hence, we aim to study salivary α amylase in disabled individuals and hypothesize that salivary α amylase could be a reliable stress biomarker in disabled individuals.
Cross-sectional study was conducted including 200 individuals grouped into controls (100) and disabled individuals (100). Disabled individuals were further grouped into physically (50) and mentally (50) disabled. Stimulated and unstimulated saliva was collected from all the participants and were investigated for salivary α-amylase by direct substrate method. The enzyme activity was analyzed using a spectrophotometer and the obtained were analyzed statistically.
Salivary α-amylase showed significant difference between controls and disabled group (p = 0.000). Salivary α-amylase was least in the mentally disabled group compared to physically disabled group. Age related comparison of salivary α-amylase was higher in physically disabled whereas gender related comparison showed females in physically disabled group to be more affected than controls.
Saliva, the easy and most researched fluid, is rich in salivary α-amylase. Salivary α-amylase is a potential biomarker to assess stress. Further diagnostic studies are required to know the salivary changes and their influence on individual general health status.
每个人在日常生活活动中都会面临压力。在疫情期间，大多数人都经历过多次抑郁、焦虑和失眠。有报道称压力会引发多种医学和牙科问题，而研究和调查压力的最快且最简单的方法是通过体液，主要是唾液。因此，我们旨在研究残疾个体的唾液α淀粉酶，并假设唾液α淀粉酶可能是残疾个体中一种可靠的压力生物标志物。
进行了一项横断面研究，包括200名个体，分为对照组（100名）和残疾个体（100名）。残疾个体进一步分为身体残疾组（50名）和精神残疾组（50名）。从所有参与者中收集刺激唾液和非刺激唾液，并通过直接底物法检测唾液α淀粉酶。使用分光光度计分析酶活性，并对所得结果进行统计学分析。
唾液α淀粉酶在对照组和残疾组之间存在显著差异（p = 0.000）。与身体残疾组相比，精神残疾组的唾液α淀粉酶含量最低。身体残疾组中唾液α淀粉酶与年龄相关的比较更高，而与性别相关的比较显示，身体残疾组中的女性比对照组受影响更大。
唾液是一种易于获取且研究较多的体液，富含唾液α淀粉酶。唾液α淀粉酶是评估压力的潜在生物标志物。需要进一步的诊断研究来了解唾液变化及其对个体总体健康状况的影响。
