Ding Hui, Hu Zhijuan, Yu Liyan, Ma Zhongjun, Ma Xiaoqiong, Chen Zhe, Wang Dan, Zhao Xiaofeng
Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
Institute of Marine Biology & Natural Products, Ocean College, Zhejiang University, Zijingang Campus, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
Steroids. 2014 Aug;86:32-8. doi: 10.1016/j.steroids.2014.04.015. Epub 2014 May 2.
In the present study, the EtOAc extract of the persistent calyx of Physalis angulata L. var. villosa Bonati (PA) was tested for its potential quinone reductase (QR) inducing activity with glutathione (GSH) as the substrate using an UPLC-ESI-MS method. The result revealed that the PA had electrophiles that could induce quinone reductase (QR) activity, which might be attributed to the modification of the highly reactive cysteine residues in Keap1. Herein, three new withanolides, compounds 3, 6 and 7, together with four known withanolides, compounds 1, 2, 4 and 5 were isolated from PA extract. Their structures were determined by spectroscopic techniques, including (1)H-, (13)C NMR (DEPT), and 2D-NMR (HMBC, HMQC, (1)H, (1)H-COSY, NOESY) experiments, as well as by HR-MS. All the seven compounds were tested for their QR induction activities towards mouse hepa 1c1c7 cells.
在本研究中，采用超高效液相色谱-电喷雾电离质谱法，以谷胱甘肽（GSH）为底物，对毛酸浆（Physalis angulata L. var. villosa Bonati，PA）宿萼的乙酸乙酯提取物的潜在醌还原酶（QR）诱导活性进行了测试。结果表明，PA含有可诱导醌还原酶（QR）活性的亲电试剂，这可能归因于对Keap1中高反应性半胱氨酸残基的修饰。在此，从PA提取物中分离出三种新的维A醇类化合物，即化合物3、6和7，以及四种已知的维A醇类化合物，即化合物1、2、4和5。通过光谱技术，包括（1）H-、（13）C核磁共振（DEPT）和二维核磁共振（HMBC、HMQC、（1）H、（1）H-COSY、NOESY）实验以及高分辨质谱，确定了它们的结构。对所有七种化合物针对小鼠肝癌1c1c7细胞的QR诱导活性进行了测试。
